Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs is widely used in clinical practice to optimise therapy, but it is limited by technical problems and cost considerations. The aim of the present study was: 1) to validate a chromatographic method for the concomitant determination of levetiracetam, lamotrigine, ethosuximide, felbamate, rufinamide, zonisamide and monohydroxycarbamazepine; 2) to develop it for dried plasma spot (DPS) assessing its reliability against the classical determination from plasma; and 3) test its clinical application. Methods: Extraction of plasma samples and DPS was done by simple precipitation. Chromatographic analysis was performed using high performance liquid chromatography with ultraviolet detection. After validation, both methods were applied for the quantification of plasma samples from patients on antiepileptic therapy. Results: Mean inter-and intra-day accuracy and precision were < 15% for all compounds both in plasma and in DPS samples. DPS samples were considered stable under tested conditions. Measurements between plasma and DPS samples appeared related (p < 0.0001). BlandAltman analysis revealed accordance in lamotrigine values with mean overestimation of concentration for DPS sample of 2.8%. Also for monohydroxycarbamazepine data the agreement was acceptable (mean overestimation of 9.2%). For levetiracetam mean difference was 7.6%, while for ethosuximide mean percentage difference was 20.6%. Conclusions: The developed methods simplify TDM of antiepileptic drugs. This is particularly relevant for the method on dried spot sample devices because it facilitates further sample handling, stability and shipments making the management of therapies in epileptic patients easier also in hospitals devoid of a dedicated laboratory.
Development and validation of a HPLC-UV method for the quantification of antiepileptic drugs in dried plasma spots / S. Baldelli, D. Cattaneo, L. Giodini, L. Baietto, G. Di Perri, A. D'Avolio, E. Clementi. - In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE. - ISSN 1434-6621. - 53:3(2015 Feb), pp. 435-444.
|Titolo:||Development and validation of a HPLC-UV method for the quantification of antiepileptic drugs in dried plasma spots|
CATTANEO, DARIO (Secondo)
CLEMENTI, EMILIO GIUSEPPE IGNAZIO (Ultimo)
|Parole Chiave:||antiepileptic drugs; dried plasma spots; high performance liquid chomatography|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||feb-2015|
|Data ahead of print / Data di stampa:||12-ago-2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1515/cclm-2014-0472|
|Appare nelle tipologie:||01 - Articolo su periodico|