Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliC<inf>Bp</inf>). Here, we present the high resolution crystal structure of FliC<inf>Bp</inf> and report the synthesis and characterization of three peptides predicted to be both B and T cell FliC<inf>Bp</inf> epitopes, by both structure-based in silico methods, and sequencebased epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.
|Titolo:||Sequence-and structure-based immunoreactive epitope discovery for Burkholderia pseudomallei flagellin|
|Parole Chiave:||Infectious Diseases; Public Health, Environmental and Occupational Health; Pharmacology, Toxicology and Pharmaceutics (all)|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||29-lug-2015|
|Digital Object Identifier (DOI):||10.1371/journal.pntd.0003917|
|Appare nelle tipologie:||01 - Articolo su periodico|