Analyses of individual biomolecules, like DNA, or DNA–protein complexes, via atomic force microscopy, require ‘gentle’ methods to immobilize DNA on surfaces, which allow the ensemble of molecules to adopt conformations dictated primarily by their physical characteristics, and which possibly permit the use of a wide selection of buffers. We show that poly-l-ornithine-coated mica is a good substrate for fast, reliable deposition of DNA for wet or dry imaging. The surface firmly secures DNA, which retains the B-form helical rise (0.34 nm bp−1). The conformations of DNA that result are reminiscent of three-dimensional random coils projected on to a plane. The contrast is good, especially in solution, and buffers with physiological concentrations of salt with or without divalent cations may be used. This is important for comparison of scanning probe microscopy results with those obtained by different techniques.
Atomic force microscopy study of DNA deposited on poly-L-ornithine coated mica / A. Podestà, L. Imperadori, W. Colnaghi, L. Finzi, P. Milani, D. Dunlap. - In: JOURNAL OF MICROSCOPY. - ISSN 0022-2720. - 215:3(2004), pp. 236-240.
|Titolo:||Atomic force microscopy study of DNA deposited on poly-L-ornithine coated mica|
PODESTA', ALESSANDRO (Primo)
MILANI, PAOLO (Penultimo)
|Parole Chiave:||Atomic force microscopy; DNA on surfaces; Substrate functionalization|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
Settore FIS/03 - Fisica della Materia
|Data di pubblicazione:||2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.0022-2720.2004.01372.x|
|Appare nelle tipologie:||01 - Articolo su periodico|