The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K<sup>+</sup>) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K<sup>+</sup> channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K<sup>+</sup> channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K<sup>+</sup> selectivity and high single-channel conductance, but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K<sup>+</sup> equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.
Engineering of a light-gated potassium channel / C. Cosentino, L. Alberio, S. Gazzarrini, M. Aquila, E. Romano, S. Cermenati, P. Zuccolini, J. Petersen, M. Beltrame, J.L. Van Etten, J.M. Christie, G. Thiel, A. Moroni. - In: SCIENCE. - ISSN 0036-8075. - 348:6235(2015 May 08), pp. 707-710. [10.1126/science.aaa2787]
Titolo: | Engineering of a light-gated potassium channel | |
Autori: | COSENTINO, CRISTIAN (Primo) ALBERIO, LAURA SILVIA (Secondo) MORONI, ANNA (Ultimo) | |
Parole Chiave: | chloride channel; ion channels; K+ channel; channelrhodopsin; domain; LOV | |
Settore Scientifico Disciplinare: | Settore BIO/04 - Fisiologia Vegetale Settore BIO/18 - Genetica Settore BIO/11 - Biologia Molecolare Settore BIO/09 - Fisiologia Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin) | |
Data di pubblicazione: | 8-mag-2015 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1126/science.aaa2787 | |
Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
File | Descrizione | Tipologia | Licenza | |
---|---|---|---|---|
Science-2015-Cosentino-707-10.pdf | Publisher's version/PDF | Administrator Richiedi una copia |