Previous evidence showed mutations of the neurofibromin type 2 gene (Nf2), encoding the tumor suppressor protein merlin, in sporadic and vestibular schwannomas affecting Schwann cells (SC). Accordingly, efforts have been addressed to identify possible factors, even environmental, that may regulate neurofibromas growth. In this context, we investigated the exposure of SC to an electromagnetic field (EMF), which is an environmental issue modulating biological processes. Here we show that SC exposed to 50 Hz EMFs change their morphology, proliferation, migration and myelinating capability. In these cells merlin is downregulated, leading to activation of two intracellular signaling pathways, ERK/AKT and Hippo. Interestingly, SC change their phenotype toward a proliferative/migrating state, which in principle may be pathologically relevant for schwannoma development.

Tumor suppressor Nf2/merlin drives Schwann cell changes following electromagnetic field exposure through Hippo-dependent mechanisms / A. Colciago, S. Melfi, G. Giannotti, V. Bonalume, M. Ballabio, L. Caffino, F. Fumagalli, V. Magnaghi. - In: CELL DEATH DISCOVERY. - ISSN 2058-7716. - 1(2015 Sep 07), pp. 15021.1-15021.10. [10.1038/cddiscovery.2015.21]

Tumor suppressor Nf2/merlin drives Schwann cell changes following electromagnetic field exposure through Hippo-dependent mechanisms

A. Colciago
Primo
;
S. Melfi
Secondo
;
G. Giannotti;V. Bonalume;M. Ballabio;L. Caffino;F. Fumagalli
Penultimo
;
V. Magnaghi
Ultimo
2015-09-07

Abstract

Previous evidence showed mutations of the neurofibromin type 2 gene (Nf2), encoding the tumor suppressor protein merlin, in sporadic and vestibular schwannomas affecting Schwann cells (SC). Accordingly, efforts have been addressed to identify possible factors, even environmental, that may regulate neurofibromas growth. In this context, we investigated the exposure of SC to an electromagnetic field (EMF), which is an environmental issue modulating biological processes. Here we show that SC exposed to 50 Hz EMFs change their morphology, proliferation, migration and myelinating capability. In these cells merlin is downregulated, leading to activation of two intracellular signaling pathways, ERK/AKT and Hippo. Interestingly, SC change their phenotype toward a proliferative/migrating state, which in principle may be pathologically relevant for schwannoma development.
Settore BIO/09 - Fisiologia
Settore BIO/14 - Farmacologia
Settore MED/04 - Patologia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/313774
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