We studied the human immunodeficiency virus type 1 phenotypic and genotypic profiles of a dual drug-resistant isolate (isolate 14aPost-DR) selected for zidovudine (ZDV) and lamivudine (3TC) resistance and then cultured in the presence of 3TC and a protease inhibitor: indinavir (IDV), ritonavir, or KNI-272. The IDV-treated virus was highly resistant to 3TC, ZDV, and IDV and accumulated protease mutations at positions M46I and V82F. A change from alanine to valine was observed in 4 of 10 clones in the P2 position of the p7-p1 Gag-protease cleavage site, linked to position M46I in the dominant viral quasispecies. Previous 3TC resistance did not impair the development of additional mutations in the protease and Gag-protease cleavage regions.
Titolo: | In vitro evolution of the human immunodeficiency virus type 1 gag-protease region and maintenance of reverse transcriptase resistance following prolonged drug exposure |
Autori: | LA SETA CATAMANCIO, SIMONA (Primo) GALLI, MASSIMO (Penultimo) RUSCONI, STEFANO (Ultimo) |
Settore Scientifico Disciplinare: | Settore MED/17 - Malattie Infettive |
Data di pubblicazione: | mar-2001 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | 10.1128/JCM.39.3.1124-1129.2001 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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