1. NCC27 is a nuclear chloride ion channel, identified in the PMA-activated U937 human monocyte cell line. NCC27 mRNA is expressed in virtually all cells and tissues and the gene encoding NCC27 is also highly conserved. Because of these factors, we have examined the hypothesis that NCC27 is involved in cell cycle regulation. 2. Electrophysiological studies in Chinese hamster ovary (CHO-K1) cells indicated that NCC27 chloride conductance varied according to the stage of the cell cycle, being expressed only on the plasma membrane of cells in G2/M phase. 3. We also demonstrate that Cl- ion channel blockers known to block NCC27 led to arrest of CHO-K1 cells in the Q2/M stage of the cell cycle, the same stage at which this ion channel is selectively expressed on the plasma membrane. 4. These data strongly support the hypothesis that NCC27 is involved, in some as vet undetermined manner, in regulation of the cell cycle.

The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle / S.M. Valenzuela, M. Mazzanti, R.F. Tonini, M.R. Qiu, K. Warton, E.A. Musgrove, T.J. Campbell, S.N. Breit. - In: THE JOURNAL OF PHYSIOLOGY. - ISSN 0022-3751. - 529:3(2000), pp. 541-552. [10.1111/j.1469-7793.2000.00541.x]

The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle

M. Mazzanti
Secondo
;
R.F. Tonini;
2000

Abstract

1. NCC27 is a nuclear chloride ion channel, identified in the PMA-activated U937 human monocyte cell line. NCC27 mRNA is expressed in virtually all cells and tissues and the gene encoding NCC27 is also highly conserved. Because of these factors, we have examined the hypothesis that NCC27 is involved in cell cycle regulation. 2. Electrophysiological studies in Chinese hamster ovary (CHO-K1) cells indicated that NCC27 chloride conductance varied according to the stage of the cell cycle, being expressed only on the plasma membrane of cells in G2/M phase. 3. We also demonstrate that Cl- ion channel blockers known to block NCC27 led to arrest of CHO-K1 cells in the Q2/M stage of the cell cycle, the same stage at which this ion channel is selectively expressed on the plasma membrane. 4. These data strongly support the hypothesis that NCC27 is involved, in some as vet undetermined manner, in regulation of the cell cycle.
Animals; Cell Size; Electric Conductivity; Multigene Family; Intracellular Membranes; Ion Channels; Gene Expression; Chlorides; Electrophysiology; Conserved Sequence; Transfection; Mitosis; Anthracenes; Cell Membrane; CHO Cells; G2 Phase; Glycolates; Cell Cycle; Chloride Channels; Cricetinae
Settore BIO/09 - Fisiologia
2000
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/30262
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