We have designed and synthesised a new organometallic molecule containing three ferrocene groups for use as a highly sensitive electro-chemical marker in biological assays. This trisferrocene derivative was conjugated to different PNA monomers, and the electrochemical activities of the conjugates were extensively investigated in organic solvents, in view of their potential diagnostic applications. The results showed that the introduction of a trisferrocene unit on the PNA monomer triples the current signal in comparison with the monoferrocene-labelled one. Despite their greater molecular complexity, trisferrocene-conjugated PNA monomers are even more electro-chemically active than the reference ferrocene. By using differential pulse voltammetry (DPV), the detection limit can reach 10(M)(-8) in acetonitrile solution. These results are a good premise for the use of the trisferrocene unit as an effective electrochemical probe for biomolecules.
A new triferrocenyl-tris(hydroxymethyl)aminomethane derivative as a highly sensitive electrochemical marker of biomolecules: Application to the labelling of PNA monomers and their electrochemical characterization / C. Baldoli, C. Rigamonti, S. Maiorana, E. Licandro, L. Falciola, P.R. Mussini. - In: CHEMISTRY-A EUROPEAN JOURNAL. - ISSN 0947-6539. - 12:15(2006), pp. 4091-4100.
|Titolo:||A new triferrocenyl-tris(hydroxymethyl)aminomethane derivative as a highly sensitive electrochemical marker of biomolecules: Application to the labelling of PNA monomers and their electrochemical characterization|
RIGAMONTI, CLARA (Secondo)
FALCIOLA, LUIGI (Penultimo)
MUSSINI, PATRIZIA ROMANA (Ultimo)
|Parole Chiave:||bioorganometallic chemistry ; electrochemistry ; ferrocene; metallocenes ; peptide nucleic acids|
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
Settore CHIM/01 - Chimica Analitica
|Data di pubblicazione:||2006|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/chem.200501466|
|Appare nelle tipologie:||01 - Articolo su periodico|