We have designed and synthesised a new organometallic molecule containing three ferrocene groups for use as a highly sensitive electro-chemical marker in biological assays. This trisferrocene derivative was conjugated to different PNA monomers, and the electrochemical activities of the conjugates were extensively investigated in organic solvents, in view of their potential diagnostic applications. The results showed that the introduction of a trisferrocene unit on the PNA monomer triples the current signal in comparison with the monoferrocene-labelled one. Despite their greater molecular complexity, trisferrocene-conjugated PNA monomers are even more electro-chemically active than the reference ferrocene. By using differential pulse voltammetry (DPV), the detection limit can reach 10(M)(-8) in acetonitrile solution. These results are a good premise for the use of the trisferrocene unit as an effective electrochemical probe for biomolecules.

A new triferrocenyl-tris(hydroxymethyl)aminomethane derivative as a highly sensitive electrochemical marker of biomolecules: Application to the labelling of PNA monomers and their electrochemical characterization / C. Baldoli, C. Rigamonti, S. Maiorana, E. Licandro, L. Falciola, P.R. Mussini. - In: CHEMISTRY-A EUROPEAN JOURNAL. - ISSN 0947-6539. - 12:15(2006), pp. 4091-4100.

A new triferrocenyl-tris(hydroxymethyl)aminomethane derivative as a highly sensitive electrochemical marker of biomolecules: Application to the labelling of PNA monomers and their electrochemical characterization

C. Rigamonti
Secondo
;
S. Maiorana;E. Licandro;L. Falciola
Penultimo
;
P.R. Mussini
Ultimo
2006

Abstract

We have designed and synthesised a new organometallic molecule containing three ferrocene groups for use as a highly sensitive electro-chemical marker in biological assays. This trisferrocene derivative was conjugated to different PNA monomers, and the electrochemical activities of the conjugates were extensively investigated in organic solvents, in view of their potential diagnostic applications. The results showed that the introduction of a trisferrocene unit on the PNA monomer triples the current signal in comparison with the monoferrocene-labelled one. Despite their greater molecular complexity, trisferrocene-conjugated PNA monomers are even more electro-chemically active than the reference ferrocene. By using differential pulse voltammetry (DPV), the detection limit can reach 10(M)(-8) in acetonitrile solution. These results are a good premise for the use of the trisferrocene unit as an effective electrochemical probe for biomolecules.
bioorganometallic chemistry ; electrochemistry ; ferrocene; metallocenes ; peptide nucleic acids
Settore CHIM/06 - Chimica Organica
Settore CHIM/01 - Chimica Analitica
http://www3.interscience.wiley.com/cgi-bin/fulltext/112510362/PDFSTART
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/30169
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