Concomitantly to the obesity epidemic, nonalcoholic fatty liver disease (NAFLD) has become the leading cause of liver disease in children. NAFLD encompasses a spectrum of histological damage ranging from simple steatosis to nonalcoholic steatohepatitis (NASH), with possible progression to cirrhosis. There is growing evidence that the immune system plays a pivotal role in the initiation and progression to NASH but the cellular nature of the hepatic inflammation is still unknown. The present study includes 34 children with biopsy-proven NAFLD. Liver damage was evaluated by the NAFLD activity score (NAS), and the inflammatory infiltrate was characterized by immunohistochemistry for CD45, CD3 and CD163 which are markers of leukocytes, T cells and activated Kupffer cells/macrophages, respectively. Our results have shown that CD45(+) (P<0.0001) and CD163(+) (P<0.0001) cells were markedly increased in children with severe histological activity (NAS >= 5) compared to children with lower activity (NAS<5), whereas CD3(+) cells were significantly lower (P<0.01) in children with severe histological activity. There was a significant association between the numbers of CD45(+), CD3(+) and CD163(+) cells, regarding both the portal tract and liver lobule, and the severity of steatosis, ballooning and fibrosis (P<0.01). These data suggest that the severity and composition of the inflammatory infiltrate correlate with steatosis and the severity of disease in children with NAFLD. Moreover, a decrease in CD3(+) cells may be involved in the pathogenesis of liver damage. Future studies should evaluate whether it can predict the progression of liver disease independently of established histological scores.
|Titolo:||Markers of activated inflammatory cells correlate with severity of liver damage in children with nonalcoholic fatty liver disease|
|Parole Chiave:||nonalcoholic fatty liver disease; nonalcoholic steatohepatitis; Kupffer cells; CD3; CD45; CD163; children|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||2012|
|Digital Object Identifier (DOI):||10.3892/ijmm.2012.965|
|Appare nelle tipologie:||01 - Articolo su periodico|