The objective of this study was to monitor with thermal imaging the surface temperature of mice within the first 5 days of life, to highlight any significant differences in skin temperature between the heterozygous and homozygous mice. The subjects will then be monitored with thermographic technique until weaning (21st day of age) to analyse the evolution of body temperature during the development of the nest and the acquisition of full thermoregulatory capacity.[1‐2] The experimental phase, lasting three months, was held at the Institute of Pharmacological Research "Mario Negri" in Milan. In the SPF facility, the temperature and relative humidity are kept constant (22 ± 2°C, 55 ± 10%), the lighting is set to 12 hours of light and 12 hours of dark(7:00 to 19:00), the air is filtered with HEPA filters, and each object is autoclaved or Peroxide to ensure the SPF status of the animal. Were used: Cages: No.3 IVC ventilated under positive pressure, air changes at 75 hour. Mice: n°3 female Hsd: Nude‐Foxn1nu athymic (nu / +) of 6 weeks of age. n° 3 males Hsd: athymic Nude‐Foxn1nu (nu / nu) of 6 weeks of age. Permissions: Ministerial Committee for experimentation. Litter: corn in cob autoclaved, 150g per cage. The bedding is changed every 2 weeks. Food: Harlan Teklad Global Diet 2018S reproduction/maintenance. Feeding ad libitum. Water: autoclaved. Administration in bottles ad libitum. Laminar Flow Hood: CS5 changing station. Thermocamera: AVIO TVS500 with uncooled microbolometer detector. Software: GORATEC Thermography Suite. In the first hours of life the animals disperse more heat despite the successful containment of the nest, and this aspect indicative, assumed as an index of increased vulnerability, has a delicate role in nature for the survival of the species itself. With decreasing temperature, the set of all metabolic processes decrease in intensity. Graphically it would seem that the thermogenic processes are activated only for the first few seconds of removal from the offspring of parents. They appear instead more and more active as the hours passed, as evidenced by the data reported on the subjects "3‐5 days". The thermographic analysis of the early life stages of nude mice has demonstrated in a concrete theoretical concepts typical of younger individuals. It is necessary to develop a new research protocol to improve the data analysis cencerning the different temperature in the nest to demonstrate the changes as regards the growing of animals. 1. Fornasier M. Redaelli V., Tarantino A., Luzi F., Verga M., (2010). Infrared Thermography (IRT) in nude mice: an alternative methods for body temperature measurement. Atti Scand FELASA 2010, Helsinki, June 14‐17, 2010. 2. Funda D. P., Houstek J., Holub M., Kazdova L., Michaelsky m., Burysek L., Cervinkova M., Sima P., (1997), Difference in Thermoregulation between Immunocompetent ad Immunodeficient Hairless Mice Exposed to Mild Cold. Folia Microbiol 43, 5: 487‐489.

Phenotyping and evaluation of heat stress by Thermal imaging in the management of mice / M. Freschi, G. Grignaschi, F. Luzi, V. Redaelli - In: Congresso Nazionale S.I.S.Vet : abstracts[s.l] : SISVET, 2013. - pp. 265-265 (( Intervento presentato al 67. convegno Convegno Nazionale Sisvet tenutosi a Brescia nel 2013.

Phenotyping and evaluation of heat stress by Thermal imaging in the management of mice

F. Luzi
Penultimo
;
V. Redaelli
Ultimo
2013

Abstract

The objective of this study was to monitor with thermal imaging the surface temperature of mice within the first 5 days of life, to highlight any significant differences in skin temperature between the heterozygous and homozygous mice. The subjects will then be monitored with thermographic technique until weaning (21st day of age) to analyse the evolution of body temperature during the development of the nest and the acquisition of full thermoregulatory capacity.[1‐2] The experimental phase, lasting three months, was held at the Institute of Pharmacological Research "Mario Negri" in Milan. In the SPF facility, the temperature and relative humidity are kept constant (22 ± 2°C, 55 ± 10%), the lighting is set to 12 hours of light and 12 hours of dark(7:00 to 19:00), the air is filtered with HEPA filters, and each object is autoclaved or Peroxide to ensure the SPF status of the animal. Were used: Cages: No.3 IVC ventilated under positive pressure, air changes at 75 hour. Mice: n°3 female Hsd: Nude‐Foxn1nu athymic (nu / +) of 6 weeks of age. n° 3 males Hsd: athymic Nude‐Foxn1nu (nu / nu) of 6 weeks of age. Permissions: Ministerial Committee for experimentation. Litter: corn in cob autoclaved, 150g per cage. The bedding is changed every 2 weeks. Food: Harlan Teklad Global Diet 2018S reproduction/maintenance. Feeding ad libitum. Water: autoclaved. Administration in bottles ad libitum. Laminar Flow Hood: CS5 changing station. Thermocamera: AVIO TVS500 with uncooled microbolometer detector. Software: GORATEC Thermography Suite. In the first hours of life the animals disperse more heat despite the successful containment of the nest, and this aspect indicative, assumed as an index of increased vulnerability, has a delicate role in nature for the survival of the species itself. With decreasing temperature, the set of all metabolic processes decrease in intensity. Graphically it would seem that the thermogenic processes are activated only for the first few seconds of removal from the offspring of parents. They appear instead more and more active as the hours passed, as evidenced by the data reported on the subjects "3‐5 days". The thermographic analysis of the early life stages of nude mice has demonstrated in a concrete theoretical concepts typical of younger individuals. It is necessary to develop a new research protocol to improve the data analysis cencerning the different temperature in the nest to demonstrate the changes as regards the growing of animals. 1. Fornasier M. Redaelli V., Tarantino A., Luzi F., Verga M., (2010). Infrared Thermography (IRT) in nude mice: an alternative methods for body temperature measurement. Atti Scand FELASA 2010, Helsinki, June 14‐17, 2010. 2. Funda D. P., Houstek J., Holub M., Kazdova L., Michaelsky m., Burysek L., Cervinkova M., Sima P., (1997), Difference in Thermoregulation between Immunocompetent ad Immunodeficient Hairless Mice Exposed to Mild Cold. Folia Microbiol 43, 5: 487‐489.
Thermography; heat stress; mouse
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore AGR/20 - Zoocolture
2013
Book Part (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/295420
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