We investigated the effect of a single administration of recombinant human erythropoietin (rhEPO) on the preservation of the ventral white matter of rats at four weeks after contusive spinal cord injury (SCI), a time at which functional recovery is significantly improved in comparison to the controls (Gorio et al., 2002, 2005). Specifically, we examined, by morphological and cytochemical methods combined with light, confocal and electron microscopy, i) myelin preservation, ii) activation of adult oligodendrocyte progenitors (OPCs) identified for the expression of NG2 transmembrane proteoglycan, iii) changes in the amount of the chondroitin sulfate proteoglycans neurocan, versican and phosphacan and of their glycosaminoglycan component labeled with Wisteria floribunda lectin, and iv) ventral horn density of the serotonergic plexus as a marker of descending motor control axons. Injured rats received either saline or a single dose of rhEPO within 30 minutes after SCI. The results showed that the significant improvement of functional outcome observed in rhEPO-treated rats was associated with a better preservation of myelin in the ventral white matter. Moreover, the significant increase of both the number of NG2-positive OPCs and the labeling for Nogo-A, a marker of differentiated oligodendrocytes, suggested that rhEPO treatment could result in the generation of new myelinating oligodendrocytes. Sparing of fiber tracts in the ventral white matter was confirmed by the increased density of the serotonergic plexus around motor neurons. As for chondroitin sulfate proteoglycans, only phosphacan, increased in saline-treated rats, returned to normal levels in rhEPO group, probably reflecting a better maintenance of glial-axolemmal relationships along nerve fibers. In conclusion, this investigation expands previous studies supporting the pleiotropic neuroprotective effect of rhEPO on secondary degenerative response and its therapeutic potential for the treatment of SCI and confirms that the preservation of the ventral white matter, which contains descending motor pathways, may be critical for limiting functional deficit.
Erythropoietin-mediated preservation of the white matter in rat spinal cord injury / L. Vitellaro Zuccarello, S. Mazzetti, L. Madaschi, P. Bosisio, A. Gorio, S. De Biasi. - In: NEUROSCIENCE. - ISSN 0306-4522. - 144:3(2007), pp. 865-877.
|Titolo:||Erythropoietin-mediated preservation of the white matter in rat spinal cord injury|
VITELLARO ZUCCARELLO, LAURA (Primo)
MAZZETTI, SAMANTA (Secondo)
GORIO, ALFREDO (Penultimo)
DE BIASI, SILVIA (Ultimo)
|Parole Chiave:||chondroitin sulfate proteoglycans; NG2; Nogo-A; oligodendrocytes; precursor cells; serotonin|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
Settore BIO/14 - Farmacologia
Settore BIO/16 - Anatomia Umana
|Data di pubblicazione:||2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.neuroscience.2006.10.023|
|Appare nelle tipologie:||01 - Articolo su periodico|