Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.

Amplified fragment lenght polymorphism analysis of Listeria monocytogenes by experionTM automated microfluidic electrophoresis / M.L. Sammarco, M.Vitullo, M. Tamburro, M. Pontello, G. Ripabelli. - In: JOURNAL OF MICROBIOLOGICAL METHODS. - ISSN 0167-7012. - 87:1(2011 Oct), pp. 119-124. [10.1016/j.mimet.2011.07.011]

Amplified fragment lenght polymorphism analysis of Listeria monocytogenes by experionTM automated microfluidic electrophoresis

M. Pontello;
2011

Abstract

Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.
Agarose gel electrophoresis; Chip-based microfluidic electrophoresis; Listeria monocytogenes; Molecular epidemiology; SAFLP
Settore MED/42 - Igiene Generale e Applicata
Settore MED/07 - Microbiologia e Microbiologia Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/292006
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