Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.
|Titolo:||Amplified fragment lenght polymorphism analysis of Listeria monocytogenes by experionTM automated microfluidic electrophoresis|
|Parole Chiave:||Agarose gel electrophoresis; Chip-based microfluidic electrophoresis; Listeria monocytogenes; Molecular epidemiology; SAFLP|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Data di pubblicazione:||ott-2011|
|Digital Object Identifier (DOI):||10.1016/j.mimet.2011.07.011|
|Appare nelle tipologie:||01 - Articolo su periodico|