Background Detection of viral DNA in dried blood spots using the Guthrie card (DBS test) is a reliable and practical method of diagnosing congenital cytomegalovirus (CMV) infection. The test lends itself to epidemiological studies to establish the prevalence of the infection, but also to neonatal screening for secondary prevention of sequelae. These applications would be facilitated if it were possible to use smaller samples and do the test on pools of individual cases. Objective To ascertain whether doing the test on smaller, pooled samples still accurately identifies neonates with congenital CMV infection. Study design We tested DBS from : (A) 39 laboratory reference cases; (B) 156 neonates suspected of having congenital CMV infection; (C) 119 children examined for the retrospective diagnosis of congenital CMV; (D) mock specimens prepared with known amounts of viral DNA. Results The test using only one third of the usual amount of dried blood was 100% sensitive and specific compared to the standard DBS test (A) and to viral isolation (A and B). Pools of three single cases gave the same results as viral isolation (B) and the small-sample test (B and C). All the versions of the test gave a detection limit of 400 copies/mL. Conclusions The modified procedure can accurately diagnose congenital CMV infection. It achieves savings in both the patient material and the costs of testing.
Modification of CMV DNA detection from dried blood spots for diagnosing congenital CMV infection / S. Binda, M.S. Caroppo, P. Didò, V. Primache, L. Veronesi, A. Calvario, A. Piana, M. Barbi. - In: JOURNAL OF CLINICAL VIROLOGY. - ISSN 1386-6532. - 30:3(2004 Jul), pp. 276-279.
|Titolo:||Modification of CMV DNA detection from dried blood spots for diagnosing congenital CMV infection|
BINDA, SANDRO (Primo)
CAROPPO, MARIA SIMONA (Secondo)
BARBI, MARIA (Ultimo)
|Parole Chiave:||Cytomegalovirus ; congenital infection ; Guthrie card ; filter paper ; screening ; PCR|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
|Data di pubblicazione:||lug-2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jcv.2003.11.012|
|Appare nelle tipologie:||01 - Articolo su periodico|