Although the neonatal sinus node beats at a faster rate than the adult, when a sodium current (INa) present in the newborn is blocked, the spontaneous rate is slower in neonatal myocytes than in adult myocytes. This suggests a possible functional substitution of INa by another current during development. We used ruptured [T-type calcium current (ICa,T)] and perforated [L-type calcium current (ICa,L)] patch clamps to study developmental changes in calcium currents in sinus node cells from adult and newborn rabbits. ICa,T density did not differ with age, and no significant differences were found in the voltage dependence of activation or inactivation. ICa,L density was lower in the adult than newborn (12.1 6 1.4 vs. 17.6 6 2.5 pA/pF, P 5 0.049). However, activation and inactivation midpoints were shifted in opposite directions, reducing the potential contribution during late diastolic depolarization in the newborn (activation midpoints 217.3 6 0.8 and 222.3 6 1.4 mV in the newborn and adult, respectively, P 5 0.001; inactivation midpoints 233.4 6 1.4 and 228.3 6 1.7 mV for the newborn and adult, respectively, P 5 0.038). Recovery of ICa,L from inactivation was also slower in the newborn. The results suggest that a smaller but more negatively activating and rapidly recovering ICa,L in the adult sinus node may contribute to the enhanced impulse initiation at this age in the absence of INa.
|Titolo:||L-type but not T-type calcium current changes during postnatal development in rabbit sinoatrial node|
DI FRANCESCO, DARIO (Secondo)
|Parole Chiave:||Automaticity; Ca current; Development; Diastolic depolarization|
|Settore Scientifico Disciplinare:||Settore BIO/09 - Fisiologia|
|Data di pubblicazione:||2001|
|Appare nelle tipologie:||01 - Articolo su periodico|