Nowadays, several screening strategies are available to prevent cervical cancer, but inadequate resources, sociocultural barriers, and sampling issues impede their success in low-income countries. To overcome these issues, this study aimed to evaluate the performance of human papillomavirus (HPV) testing from dried urine spots (DUS). Eighty-eight urine samples (including 56 HPV DNA positive specimens) were spotted on filter paper, dried, and stored in paper-bags. HPV DNA was detected from the DUS after 1 week and 4 weeks of storage using a polymerase chain reaction (PCR) assay. The sensitivity, specificity, and concordance of the DUS-based HPV test were evaluated by comparing the results with those of HPV testing on fresh urine samples as the gold standard. The sensitivity of the test was 98.21% (95% CI: 90.56–99.68) for DUS stored for 1 week and 96.42% (95% CI: 87.88–99.01) for DUS stored for 4 weeks. The specificity was 100% (95% CI: 89.28–100) at both time points. The concordance between DUS and fresh urine HPV testing was “almost perfect” using the κ statistic. These preliminary data suggest that a DUS-based assay could bypass sociocultural barriers and sampling issues and therefore could be a suitable, effective tool for epidemiological surveillance and screening programs, especially in low-income countries.
HPV testing from dried urine spots as a tool for cervical cancer screening in low-income countries / E.R. Frati, M. Martinelli, E. Fasoli, D. Colzani, S. Bianchi, S. Binda, P. Olivani, E. Tanzi. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6141. - (2015), pp. 283036.1-283036.5.
|Titolo:||HPV testing from dried urine spots as a tool for cervical cancer screening in low-income countries|
FRATI, ELENA ROSANNA (Primo)
MARTINELLI, MARIANNA (Secondo)
TANZI, ELISABETTA (Ultimo)
|Parole Chiave:||Human-papillomavirus DNA; filter-paper; samples; infection; amplification; cytology|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
Settore MED/07 - Microbiologia e Microbiologia Clinica
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1155/2015/283036|
|Appare nelle tipologie:||01 - Articolo su periodico|