Hepatitis E Virus (HEV) is the causative agent of an acute and self-limited form of hepatitis. The virus is transmitted by the faecal–oral route and is a major cause of viral hepatitis in much of the developing world where it causes sporadic infections and large-scale epidemics. A simple and rapid protocol for the measurement of HEV faecal shedding by a real-time polymerase chain reaction (PCR) with the SYBR Green method on a LightCycler instrument, is described. After only 3 h the real-time quantitative PCR method detected 10 molecules of HEV cDNA fragment per reaction tube and showed a high linear dynamic range of quantitation (10–106 molecules of cDNA/reaction) with a good correlation (r=−1.00). Its specificity was confirmed by assay in human faecal samples.
|Titolo:||Detection and quantitation of hepatitis E virus in human faeces by real-time quantitative PCR|
|Parole Chiave:||Hepatitis E virus; LightCycler; Real-time PCR|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
|Data di pubblicazione:||15-giu-2004|
|Digital Object Identifier (DOI):||10.1016/j.jviromet.2004.01.025|
|Appare nelle tipologie:||01 - Articolo su periodico|