To understand the mechanisms which enable the nucleus to function under low water potential, the morphology and biochemistry of potato cell nuclei were studied. Conformational modifications were observed in the chromatin of nuclei of cells growing under low water potential. These modifications include a higher number of heterochromatic centres, enlargement of the nuclear diameter, and a different accessibility of DNA to the action of restriction enzymes. Biochemical analyses showed that these chromatin modifications may coincide with quantitative and qualitative variations of several nuclear proteins, some of which may belong to the dehydrin family. We especially focussed our attention on a 45-kDa protein that is heat-stable and is recognised by an antibody raised against the conserved domain of dehydrins. The survival of potato cells in an environment where water availability is low may die p end on several simultaneous events regarding the nucleus. The accumulation in the nucleus of specific proteins such as dehydrins could be required to stabilise the chromatin by means of their molecule-salvation action. Further studies are in progress to check whether or not variations in chromatin organisation may be one of the numerous traits that a cell must acquire to become water-stress resistant.
|Titolo:||Modification of chromatin organisation at low water potential in cultured cells of Solanum tuberosum: possible involvement of dehydrins|
ONELLI, ELISABETTA (Penultimo)
PATRIGNANI, GIUSEPPE (Ultimo)
|Parole Chiave:||cell proliferation ; chromatin organisation ; dehydrins ; nuclei ; Solanum tuberosum|
|Settore Scientifico Disciplinare:||Settore AGR/02 - Agronomia e Coltivazioni Erbacee|
|Data di pubblicazione:||2002|
|Appare nelle tipologie:||01 - Articolo su periodico|