Cells modify their volume in response to changes in osmotic pressure but it is usually assumed that other active shape variations do not involve significant volume fluctuations. Here we report experiments demonstrating that water transport in and out of the cell is needed for the formation of blebs, commonly observed protrusions in the plasma membrane driven by cortex contraction. We develop and simulate a model of fluid mediated membrane-cortex deformations and show that a permeable membrane is necessary for bleb formation which is otherwise impaired. Taken together our experimental and theoretical results emphasize the subtle balance between hydrodynamics and elasticity in actively driven cell morphological changes.

Volume changes during active shape fluctuations in cells / A. Taloni, E. Kardash, O. Umut Salman, L. Truskinovsky, S. Zapperi, C.A.M. La Porta. - In: PHYSICAL REVIEW LETTERS. - ISSN 0031-9007. - 114:20(2015 May 22), pp. 208101.1-208101.5. [10.1103/PhysRevLett.114.208101]

Volume changes during active shape fluctuations in cells

S. Zapperi
Penultimo
;
C.A.M. La Porta
2015

Abstract

Cells modify their volume in response to changes in osmotic pressure but it is usually assumed that other active shape variations do not involve significant volume fluctuations. Here we report experiments demonstrating that water transport in and out of the cell is needed for the formation of blebs, commonly observed protrusions in the plasma membrane driven by cortex contraction. We develop and simulate a model of fluid mediated membrane-cortex deformations and show that a permeable membrane is necessary for bleb formation which is otherwise impaired. Taken together our experimental and theoretical results emphasize the subtle balance between hydrodynamics and elasticity in actively driven cell morphological changes.
Settore MED/04 - Patologia Generale
Settore FIS/02 - Fisica Teorica, Modelli e Metodi Matematici
22-mag-2015
Article (author)
File in questo prodotto:
File Dimensione Formato  
PRLArxiv 2015.pdf

accesso aperto

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 3.53 MB
Formato Adobe PDF
3.53 MB Adobe PDF Visualizza/Apri
PhysRevLett_114_208101.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 756.4 kB
Formato Adobe PDF
756.4 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/272593
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 31
  • ???jsp.display-item.citation.isi??? 30
social impact