In vitro techniques can offer a rapid, repeatable and cost-effective alternative to in vivo experiments. We investigated the gas production (GP) of nine different feeds commonly used in Italian and Maltese pig farms in order to assess the use of the Ankom system in this species and to compare the in vitro microbial activity fresh or frozen inocula derived from piglet feces. Proximate and polyphenolic analyses were determined for all substrates. Fermentation proﬁles were measured according to the cumulative GP technique. Analysis of GP values revealed signiﬁcant differences in the fermentation patterns mainly due to substrate and inoculum effects. Fermentation parameters varied signiﬁcantly according to the substrate ingredients. The frozen inoculum generally led to more total GP than for fresh, with the exception of hard wheat bran pellets (HWBP), pelleted pulp and mature carob. Conversely the fresh fecal inoculum had an earlier maximum time to reach maximum GP than the frozen, showing a higher maximum rate of GP in all substrates excepting for HWBP. A signiﬁcant difference in frozen and fresh inocula was found for the complete formulation. Further studies are required to conﬁrm the variation between fresh and frozen fecal inocula to a wider range of feed, including those that could have negative biological effects.
In vitro fermentation of feed ingredients by fresh or frozen pig fecal inocula / G. Pastorelli, M. Faustini, E. Attard. - In: ANIMAL SCIENCE JOURNAL. - ISSN 1740-0929. - 85:6(2014 Jun), pp. 690-697.
|Titolo:||In vitro fermentation of feed ingredients by fresh or frozen pig fecal inocula|
PASTORELLI, GRAZIA (Primo)
FAUSTINI, MASSIMO (Secondo)
|Parole Chiave:||fecal inocula; feeds; in vitro; inoculum preservation; piglet|
|Settore Scientifico Disciplinare:||Settore AGR/18 - Nutrizione e Alimentazione Animale|
|Data di pubblicazione:||giu-2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/asj.12192|
|Appare nelle tipologie:||01 - Articolo su periodico|