Acrosin was localized on the acrosomal membranes of boar sperm by immunofluorescence and immunoelectron microscopy. Indirect labeling of sperm head sections was performed with an Imposil (iron-dextran) antibody conjugate. The results of electron microscopy indicated a preferential deposition of the antiacrosin antibodies on the outer acrosomal membrane, although a lesser deposition was also found on the inner acrosomal membrane. No labeling was detected in the acrosomal content when such material was still present.
|Titolo:||Immunocytochemical localization of acrosin in boar spermatozoa|
BERRUTI, GIOVANNA (Secondo)
COLOMBO, ROBERTO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||1983|
|Digital Object Identifier (DOI):||10.1002/jez.1402270213|
|Appare nelle tipologie:||01 - Articolo su periodico|