Histo-cytochemistry and scanning electron microscopy of lavender glandular trichomes following conventional and microwave-assisted hydrodistillation of essential oils: a comparative study

The conventional hydrodistillation method (HD) has been compared with three different variants of the microwave-assisted hydrodistillation technique (MADH), on Lavandula anguslifolia flowers, both in terms of oil yield and effects on the glandular trichome morpho-structure. The amount and composition of essential oil extracted in 4 h by HD was similar to that obtained in only 20 min by MADH and even lower than that extracted with a steam variant (MASD) of MADH itself. Histo-cytochemical investigations and scanning electron microscopy showed that glandular trichome, after 4 h of HD, appeared swollen, with a deeply wrinkled surface and empty of essential oil, however maintaining the integrity of their cuticle. Many oil droplets were stuck to the outside of the trichome cuticle, sometimes arranged in large aggregates. After 20 min of MADH or MASD, trichomes appeared empty as well, but also particularly disrupted, or at least with holes in their head cuticle. Oil droplets were present outside the glands and in the inter-rib regions of the flower calyx, except in the case of MASD. After this extraction method, neither oil droplets nor other lipid staining were present in the samples, confirming the effectiveness of this particular MADH technique. These investigations suggest that the rapidity of MADH and MASD in extracting essential oil is mainly due to the collapse of trichome structure and the consequent direct release of its content, without which it has to slowly permeate through membranes and cuticle, as in the case of HD. Copyright