Dendritic Cells (DCs), the most potent antigenpresenting cells, play a critical role in the detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one of the best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present a potent DC-SIGN targeting device developed using gold nanoparticles functionalized with α-fucosyl-β-alanyl amide. The nanoparticles bound to cellular DC-SIGN and induced internalization as effectively as similar particles coated with comparable amounts of LewisX oligosaccharide. They were found to be neutral toward dendritic cell maturation and IL-10 production, thus envisaging a possible use as targeted imaging tools and antigen delivery devices.

Effective targeting of DC-sign by α-fucosylamide functionalized gold nanoparticles / D. Arosio, F. Chiodo, J.J. Reina, M. Marelli, S. Penadés, Y. Van Kooyk, J.J. Garcia-Vallejo, A. Bernardi. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 25:12(2014), pp. 2244-2251.

Effective targeting of DC-sign by α-fucosylamide functionalized gold nanoparticles

D. Arosio
;
J.J. Reina;M. Marelli;A. Bernardi
Ultimo
2014

Abstract

Dendritic Cells (DCs), the most potent antigenpresenting cells, play a critical role in the detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one of the best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present a potent DC-SIGN targeting device developed using gold nanoparticles functionalized with α-fucosyl-β-alanyl amide. The nanoparticles bound to cellular DC-SIGN and induced internalization as effectively as similar particles coated with comparable amounts of LewisX oligosaccharide. They were found to be neutral toward dendritic cell maturation and IL-10 production, thus envisaging a possible use as targeted imaging tools and antigen delivery devices.
Biotechnology; Organic Chemistry; carbohydrate chemistry; Immunology
Settore CHIM/06 - Chimica Organica
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/264496
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