The embryotoxic potential of chlorpyrifos (CPF) and malathion (MTN), two organ ophosphorus insecticides (OPs), was evaluated by modified Frog Embryo Teratogenesis Assay-Xenopus (FETAX). CPF and MTN were not embryolethal even at the highest concentration tested (6000 mug/l), but both exhibited a powerful teratogenicity. The probit analysis of malformed larva percentages showed a TC50 of 161.54 mug/l for CPF, and a TC50 of 2394.01 mug/l for MTN. Therefore, CPF teratogenicity was about 15 times higher than MTN. Larvae of both exposed groups were mainly affected by ventral and/or lateral tail flexure coupled with abnormal gut coiling. Histopathological diagnosis displayed abnormal myotomes and myocytes with marked hypertrophics localized at the cell extremity, probably due to a break away of myofibril extremities at the intersomitic junction level. We speculate that this muscular damage was related to inhibition of acetylcholinesterase that showed a clear concentration-response in CPF and MTN exposed larvae. The teratogenic effects of these anti-cholinesterase compounds on Xenopus laevis myogenesis suggest a possible role played by OPs on induction of congenital muscular dystrophy.
|Titolo:||Comparative teratogenicity of Chlorpyrifos and Malathion on Xenopus laevis development|
|Autori interni:||VAILATI, GIOVANNI (Penultimo)|
VISMARA, CLAUDIO ENRICO (Ultimo)
|Parole Chiave:||chlorpyrifos ; FETAX ; malathion ; muscular damage|
|Settore Scientifico Disciplinare:||Settore BIO/06 - Anatomia Comparata e Citologia|
|Data di pubblicazione:||2004|
|Appare nelle tipologie:||01 - Articolo su periodico|