A 3-year-old, male, domestic shorthaired cat was presented with a 3-day history of anorexia and depression. The cat was moderately dehydrated, had pale, slightly icteric, mucous membranes, oral ulcerations, and mild hepatosplenomegaly. A feline leukemia virus (FeLV) antigen test was positive. CBC results obtained at initial presentation included severe normocytic, normochromic, nonregenerative anemia, severe thrombocytopenia, and marked leukocytosis (&rt; 100,000/mu L) with 77% eosinophils. After 15 days of treatment with prednisone and doxycycline, the cat had persistent severe nonregenerative anemia (HCT 3.4%), thrombocytopenia (28,000/mu L), and extreme eosinophiha (total eosinophils, 123.1 X 10(3)/mu L; segmented 103.0 X 10(3)/mu L; immature 20.1 X 10(3)/mu L). Cytologic examination of aspirates from bone marrow, liver, lymph nodes, and spleen revealed a predominance of mature and immature eosinophils, many with dysplastic changes. The M:E ratio was 96.4. On histopathologic examination, multiple organs were infiltrated by eosinophilic granulocytes. Neoplastic cells in blood and bone marrow stained positive for alkaline phosphatase and were negative for myeloperoxidase, chloroacetate esterase, and alpha-naphthyl acetate esterase. On flow cytometric analysis of peripheral blood, the neoplastic cells were positive for CD11b and CD14. These findings were consistent with chronic eosinophilic leukemia. To our knowledge, this is the first report of chronic eosinophilic leukemia in a cat associated with naturally acquired FeLV infection, in which flow cytometry was used to characterize the neoplastic cells.
Chronic eosinophilic leukemia in a cat : cytochemical and immunophenotypical features / M. E. Gelain, E. Antoniazzi, W. Bertazzolo, M. Zaccolo, S. Comazzi. - In: VETERINARY CLINICAL PATHOLOGY. - ISSN 0275-6382. - 35:4(2006), pp. 454-459.
|Titolo:||Chronic eosinophilic leukemia in a cat : cytochemical and immunophenotypical features|
GELAIN, MARIA ELENA (Primo)
COMAZZI, STEFANO (Ultimo)
|Parole Chiave:||Cat; Chronic eosinophilic leukemia; Cytochemistry; Flow cytometry|
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Data di pubblicazione:||2006|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1939-165X.2006.tb00164.x|
|Appare nelle tipologie:||01 - Articolo su periodico|