A plethora of work has been dedicated to the analysis of cell behavior on substrates with ordered topographical features. However, the natural cell microenvironment is characterized by biomechanical cues organized over multiple scales. Here, randomly rough, self-affinefractal surfaces are generated out of silicon,where roughness R a and fractal dimension D f are independently controlled. The proliferation rates, the formation of adhesion structures, and the morphology of 3T3 murine fibroblasts are monitored over six different substrates. The proliferation rate is maximized on surfaces with moderate roughness (R a ∼ 40nm) and large fractal dimension (D f ∼ 2.4); whereas adhesion structures are wider and more stable on substrates with higher roughness (R a ∼ 50nm) and lower fractal dimension (D f ∼ 2.2). Higher proliferation occurson substrates exhibiting densely packed and sharp peaks, whereas more regular ridges favor adhesion. These results suggest that randomly roughtopographies can selectively modulate cell behavior.

Selective modulation of cell response on engineered fractal silicon substrates / F. Gentile, R. Medda, L. Cheng, E. Battista, P.E. Scopelliti, P. Milani, E.A. Cavalcanti-Adam, P. Decuzzi. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 3(2013), pp. 1461.1-1461.10.

Selective modulation of cell response on engineered fractal silicon substrates

P. Milani;
2013

Abstract

A plethora of work has been dedicated to the analysis of cell behavior on substrates with ordered topographical features. However, the natural cell microenvironment is characterized by biomechanical cues organized over multiple scales. Here, randomly rough, self-affinefractal surfaces are generated out of silicon,where roughness R a and fractal dimension D f are independently controlled. The proliferation rates, the formation of adhesion structures, and the morphology of 3T3 murine fibroblasts are monitored over six different substrates. The proliferation rate is maximized on surfaces with moderate roughness (R a ∼ 40nm) and large fractal dimension (D f ∼ 2.4); whereas adhesion structures are wider and more stable on substrates with higher roughness (R a ∼ 50nm) and lower fractal dimension (D f ∼ 2.2). Higher proliferation occurson substrates exhibiting densely packed and sharp peaks, whereas more regular ridges favor adhesion. These results suggest that randomly roughtopographies can selectively modulate cell behavior.
Analysis of Variance; Animals; Cell Adhesion; Cell Proliferation; Cell Shape; Fibroblasts; Mice; Microscopy, Atomic Force; Microscopy, Fluorescence; NIH 3T3 Cells; Silicon; Surface Properties; Time Factors; Fractals; Multidisciplinary
Settore FIS/03 - Fisica della Materia
2013
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/258082
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