THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and prevents transcription-associated recombination. Whether or not it has a ubiquitous role in the genome is unknown. Chromatin immunoprecipitation (ChIP)-chip studies reveal that the Hpr1 component of THO and the Sub2 RNA-dependent ATPase have genome-wide distributions at active ORFs in yeast. In contrast to RNA polymerase II, evenly distributed from promoter to termination regions, THO and Sub2 are absent at promoters and distributed in a gradual 5' → 3' gradient. This is accompanied by a genome-wide impact of THO-Sub2 deletions on expression of highly expressed, long and high G+C-content genes. Importantly, ChIP-chips reveal an over-recruitment of Rrm3 in active genes in THO mutants that is reduced by RNaseH1 overexpression. Our work establishes a genome-wide function for THO-Sub2 in transcription elongation and mRNP biogenesis that function to prevent the accumulation of transcription-mediated replication obstacles, including R-loops.

Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles / B. Gómez‐González, M. García‐Rubio, R. Bermejo, H. Gaillard, K. Shirahige, A. Marín, M. Foiani, A. Aguilera. - In: EMBO JOURNAL. - ISSN 0261-4189. - 30:15(2011 Aug 03), pp. 3106-3119.

Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles

M. Foiani
Penultimo
;
2011

Abstract

THO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and prevents transcription-associated recombination. Whether or not it has a ubiquitous role in the genome is unknown. Chromatin immunoprecipitation (ChIP)-chip studies reveal that the Hpr1 component of THO and the Sub2 RNA-dependent ATPase have genome-wide distributions at active ORFs in yeast. In contrast to RNA polymerase II, evenly distributed from promoter to termination regions, THO and Sub2 are absent at promoters and distributed in a gradual 5' → 3' gradient. This is accompanied by a genome-wide impact of THO-Sub2 deletions on expression of highly expressed, long and high G+C-content genes. Importantly, ChIP-chips reveal an over-recruitment of Rrm3 in active genes in THO mutants that is reduced by RNaseH1 overexpression. Our work establishes a genome-wide function for THO-Sub2 in transcription elongation and mRNP biogenesis that function to prevent the accumulation of transcription-mediated replication obstacles, including R-loops.
English
R-loops; replication impairment; Rrm3; THO/TREX; transcription
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Pubblicazione scientifica
3-ago-2011
IRL Press : Oxford university press
30
15
3106
3119
14
Pubblicato
Periodico con rilevanza internazionale
Pubmed
Aderisco
info:eu-repo/semantics/article
Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles / B. Gómez‐González, M. García‐Rubio, R. Bermejo, H. Gaillard, K. Shirahige, A. Marín, M. Foiani, A. Aguilera. - In: EMBO JOURNAL. - ISSN 0261-4189. - 30:15(2011 Aug 03), pp. 3106-3119.
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B. Gómez‐González, M. García‐Rubio, R. Bermejo, H. Gaillard, K. Shirahige, A. Marín, M. Foiani, A. Aguilera...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/257724
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