This work reports the feasibility of caffeic acid grafted PLGA (g-CA-PLGA) to design biodegradable sterile microspheres for the delivery of proteins. Ovalbumin (OVA) was selected as model compound because of its sensitiveness of γ-radiation. The adopted grafting procedure allowed us to obtain a material with good free radical scavenging properties, without a significant modification of Mw and Tg of the starting PLGA (Mw PLGA = 26.3 ± 1.3 kDa vs. Mw g-CA-PLGA = 22.8 ± 0.7 kDa; Tg PLGA = 47.7 ± 0.8 °C vs. Tg g-CA-PLGA = 47.4 ± 0.2 °C). By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs. EEg-CA-PLGA = 95.6% ± 2.7%). Microspheres particle size distribution ranged from 15 to 50 µm. The zeta-potential values of placebo and loaded microspheres were -25 mV and -15 mV, respectively. The irradiation of g-CA-PLGA at the dose of 25 kGy caused a less than 1% variation of Mw and the degradation patterns of the non-irradiated and irradiated microspheres were superimposable. The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres. These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs.
Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation / F. Selmin, F. Puoci, O.I. Parisi, S. Franzé, U.M. Musazzi, F. Cilurzo. - In: JOURNAL OF FUNCTIONAL BIOMATERIALS. - ISSN 2079-4983. - 6:1(2015 Jan 05), pp. 1-13-13.
|Titolo:||Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation|
SELMIN, FRANCESCA (Primo)
CILURZO, FRANCESCO (Corresponding)
|Parole Chiave:||anti-oxidant; microencapsulation; drug release; caffeic acid; grafting; γ-irradiation; microspheres; ovalbumin; poly(lactide-co-glycolide); sterilization|
|Settore Scientifico Disciplinare:||Settore CHIM/09 - Farmaceutico Tecnologico Applicativo|
|Data di pubblicazione:||5-gen-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/jfb6010001|
|Appare nelle tipologie:||01 - Articolo su periodico|