The possible relation between nitric oxide synthase (NOS) activity and neural differentiation was investigated using primary cultures of rat cerebellar granule cells differentiating in culture, NOS activity was measured in the cytosolic and particulate fractions obtained from cell homogenate, In the experimental conditions used the optimal pH for NOS activity was about 6.4, the activity being about 3-fold higher than at pH 7.4, Cerebellar granule cell differentiation was associated with marked increases in NOS activity, In undifferentiated cells the enzyme was almost evenly distributed between the cytosolic and particulate fractions, during differentiation there was a 12-fold increase in activity in the cytosolic enzyme and a 3-fold increase in the particulate one. This indicates a marked preferential enrichment of the cytosolic enzyme during differentiation. Cerebellar granule cells produced and released NO in the culture medium; NO formation being markedly higher in differentiated cells (7-12 DIC) than in undifferentiated (2-3 DIC) ones, These data demonstrate a relationship between NOS expression and NO production and the differentiation of cerebellar granule cells, supporting the notion that NO may play a role in this process. (C) 1997 Federation of European Biochemical Societies.

Behaviour of nitric oxide synthase in rat cerebellar granule cells differentiating in culture / P. Viani , P. Giussani , L. Riboni , R. Bassi , G. Tettamanti. - In: FEBS LETTERS. - ISSN 0014-5793. - 408:2(1997), pp. 131-134.

Behaviour of nitric oxide synthase in rat cerebellar granule cells differentiating in culture

P. Viani;P. Giussani;L. Riboni;R. Bassi;
1997

Abstract

The possible relation between nitric oxide synthase (NOS) activity and neural differentiation was investigated using primary cultures of rat cerebellar granule cells differentiating in culture, NOS activity was measured in the cytosolic and particulate fractions obtained from cell homogenate, In the experimental conditions used the optimal pH for NOS activity was about 6.4, the activity being about 3-fold higher than at pH 7.4, Cerebellar granule cell differentiation was associated with marked increases in NOS activity, In undifferentiated cells the enzyme was almost evenly distributed between the cytosolic and particulate fractions, during differentiation there was a 12-fold increase in activity in the cytosolic enzyme and a 3-fold increase in the particulate one. This indicates a marked preferential enrichment of the cytosolic enzyme during differentiation. Cerebellar granule cells produced and released NO in the culture medium; NO formation being markedly higher in differentiated cells (7-12 DIC) than in undifferentiated (2-3 DIC) ones, These data demonstrate a relationship between NOS expression and NO production and the differentiation of cerebellar granule cells, supporting the notion that NO may play a role in this process. (C) 1997 Federation of European Biochemical Societies.
Cerebellar granule cell; Neuronal differentiation; Nitric oxide; Nitric oxide synthase
Settore BIO/10 - Biochimica
FEBS LETTERS
Article (author)
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0014579397004067-main.pdf

non disponibili

496.98 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/249137
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 15
social impact