The mammalian oocyte nucleus or germinal vesicle (GV) exhibits characteristic chromatin configurations, which are subject to dynamic modifications through oogenesis. Aim of this review is to highlight how changes in chromatin configurations are related to both functional and structural modifications occurring in the oocyte nuclear and cytoplasmic compartments. During the long phase of meiotic arrest at the diplotene stage, the chromatin enclosed within the GV is subjected to several levels of regulation. Morphologically, the chromosomes lose their individuality and form a loose chromatin mass. The decondensed configuration of chromatin then undergoes profound rearrangements during the final stages of oocyte growth that are tightly associated with the acquisition of meiotic and developmental competence. Functionally, the discrete stages of chromatin condensation are characterized by different level of transcriptional activity, DNA methylation and covalent histone modifications. Interestingly, the program of chromatin rearrangement is not completely intrinsic to the oocyte, but follicular cells exert their regulatory actions through gap junction mediated communications and intracellular messenger dependent mechanism(s). With this in mind and since oocyte growth mostly relies on the bidirectional interaction with the follicular cells, a connection between cumulus cells gene expression profile and oocyte developmental competence, according to chromatin configuration is proposed. This analysis can help in identifying candidate genes involved in the process of oocyte developmental competence acquisition and in providing non-invasive biomarkers of oocyte health status that can have important implications in treating human infertility as well as managing breeding schemes in domestic mammals. Supported by CIG-Marie Curie Actions FP7-People (Pro-Ovum) and by NSERC Strategic Network EmbryoGENE.

Changes in Large-Scale Chromatin Structure and Function During Oogenesis : A journey in Company of Follicular Cells / A.M. Luciano, F. Franciosi, C. Dieci, V. Lodde. ((Intervento presentato al 4. convegno 4th Mammalian Embryo Genomics meeting tenutosi a Quebec City nel 2013.

Changes in Large-Scale Chromatin Structure and Function During Oogenesis : A journey in Company of Follicular Cells

A.M. Luciano
Primo
;
F. Franciosi
Secondo
;
C. Dieci
Penultimo
;
V. Lodde
Ultimo
2013

Abstract

The mammalian oocyte nucleus or germinal vesicle (GV) exhibits characteristic chromatin configurations, which are subject to dynamic modifications through oogenesis. Aim of this review is to highlight how changes in chromatin configurations are related to both functional and structural modifications occurring in the oocyte nuclear and cytoplasmic compartments. During the long phase of meiotic arrest at the diplotene stage, the chromatin enclosed within the GV is subjected to several levels of regulation. Morphologically, the chromosomes lose their individuality and form a loose chromatin mass. The decondensed configuration of chromatin then undergoes profound rearrangements during the final stages of oocyte growth that are tightly associated with the acquisition of meiotic and developmental competence. Functionally, the discrete stages of chromatin condensation are characterized by different level of transcriptional activity, DNA methylation and covalent histone modifications. Interestingly, the program of chromatin rearrangement is not completely intrinsic to the oocyte, but follicular cells exert their regulatory actions through gap junction mediated communications and intracellular messenger dependent mechanism(s). With this in mind and since oocyte growth mostly relies on the bidirectional interaction with the follicular cells, a connection between cumulus cells gene expression profile and oocyte developmental competence, according to chromatin configuration is proposed. This analysis can help in identifying candidate genes involved in the process of oocyte developmental competence acquisition and in providing non-invasive biomarkers of oocyte health status that can have important implications in treating human infertility as well as managing breeding schemes in domestic mammals. Supported by CIG-Marie Curie Actions FP7-People (Pro-Ovum) and by NSERC Strategic Network EmbryoGENE.
Settore VET/01 - Anatomia degli Animali Domestici
Changes in Large-Scale Chromatin Structure and Function During Oogenesis : A journey in Company of Follicular Cells / A.M. Luciano, F. Franciosi, C. Dieci, V. Lodde. ((Intervento presentato al 4. convegno 4th Mammalian Embryo Genomics meeting tenutosi a Quebec City nel 2013.
Conference Object
File in questo prodotto:
File Dimensione Formato  
Embryo_Genomics_2013_-_Abstract_book_01.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 987.39 kB
Formato Adobe PDF
987.39 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/248671
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact