Germ cells divide and differentiate in a unique local microenvironment under the control of somatic cells. Signals released in this niche instruct oocyte reentry into the meiotic cell cycle. Once initiated, the progression through meiosis and the associated programme of maternal messenger RNA translation are thought to be cell autonomous. Here we show that translation of a subset of maternal mRNAs critical for embryo development is under the control of somatic cell inputs. Translation of specific maternal transcripts increases in oocytes cultured in association with somatic cells and is sensitive to EGF-like growth factors that act only on the somatic compartment. In mice deficient in amphiregulin, decreased fecundity and oocyte developmental competence is associated with defective translation of a subset of maternal mRNAs. These somatic cell signals that affect translation require activation of the PI(3)K-AKT-mTOR pathway. Thus, mRNA translation depends on somatic cell cues that are essential to reprogramme the oocyte for embryo development.

Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes / J. Chen, S. Torcia, F. Xie, C. Lin, H. Cakmak, F. Franciosi, K. Horner, C. Onodera, J.S. Song, M.I. Cedars, M. Ramalho-Santos, M. Conti. - In: NATURE CELL BIOLOGY. - ISSN 1465-7392. - 15:12(2013), pp. 1415-1423. [10.1038/ncb2873]

Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes

F. Franciosi;
2013

Abstract

Germ cells divide and differentiate in a unique local microenvironment under the control of somatic cells. Signals released in this niche instruct oocyte reentry into the meiotic cell cycle. Once initiated, the progression through meiosis and the associated programme of maternal messenger RNA translation are thought to be cell autonomous. Here we show that translation of a subset of maternal mRNAs critical for embryo development is under the control of somatic cell inputs. Translation of specific maternal transcripts increases in oocytes cultured in association with somatic cells and is sensitive to EGF-like growth factors that act only on the somatic compartment. In mice deficient in amphiregulin, decreased fecundity and oocyte developmental competence is associated with defective translation of a subset of maternal mRNAs. These somatic cell signals that affect translation require activation of the PI(3)K-AKT-mTOR pathway. Thus, mRNA translation depends on somatic cell cues that are essential to reprogramme the oocyte for embryo development.
Settore VET/01 - Anatomia degli Animali Domestici
Article (author)
File in questo prodotto:
File Dimensione Formato  
2013_Chen_NatCellBiol.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.73 MB
Formato Adobe PDF
1.73 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/248669
Citazioni
  • ???jsp.display-item.citation.pmc??? 50
  • Scopus 106
  • ???jsp.display-item.citation.isi??? 100
social impact