Glioblastoma multiforme (GBM) is the most aggressive primary human brain tumor. The relatively high amount of T regulatory lymphocytes present in the tumor, contributes to the establishment of an immunosuppressive microenvironment. Samples of peripheral blood were collected from GBM patients and healthy controls and a purified population of Treg (CD4+/ CD25bright) was isolated using flow cytometric cell sorting. Treg migrating capacities toward human glioma cell line conditioned medium were evaluated through an in vitro migration test. Our data show that supernatants collected from GBM cell lines were more attractant to Treg when compared to complete standard medium. The addition of an anti-CCL2 antibody to conditioned medium decreased conditioned medium-depending Treg migration, suggesting that CCL2 (also known as Monocyte Chemoattractant Protein, MCP-1) is implicated in the process. The number of circulating CD4+/μL or Treg/μL was similar in GBM patients and controls. Specific Treg markers (FOXP3; CD127; Helios; GITR; CTLA4; CD95; CCR2, CCR4; CCR7) were screened in peripheral blood and no differences could be detected between the two populations. These data confirm that the tumor microenvironment is attractive to Treg, which tend to migrate toward the tumor region changing the immunological response. Though we provide evidence that CCL2 is implicated in Treg migration, other factors are needed as well to provide such effect. © 2013 Springer Science+Business Media New York.
Circulating T regulatory cells migration and phenotype in glioblastoma patients: An in vitro study / C. Vasco, A. Canazza, A. Rizzo, A. Mossa, E. Corsini, A. Silvani, L. Fariselli, A. Salmaggi, E. Ciusani. - In: JOURNAL OF NEURO-ONCOLOGY. - ISSN 0167-594X. - 115:3(2013 Sep 05), pp. 353-363. ((Intervento presentato al 2. convegno XV Congresso Residenziale della Associazione Italiana di Neuro-Oncologia, Fiuggi 2010; SIN, 2012.
|Titolo:||Circulating T regulatory cells migration and phenotype in glioblastoma patients: An in vitro study|
|Parole Chiave:||CCL2; Glioblastoma multiforme; Irradiation; Treg|
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
|Data di pubblicazione:||5-set-2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s11060-013-1236-x|
|Appare nelle tipologie:||01 - Articolo su periodico|