Mitochondria, as recently suggested [1], might be involved in Fe sensing and signalling pathways in plant cells. For a deep understanding of the role of these organelles in the Fe deficiency-mediating responses in plant cells, it is crucial to provide a full overview of their changes occurring under Fe-limited conditions. The aim of this work is to characterize ultrastructural as well as biochemical changes of leaf mitochondria in Fe-deficient Cucumis sativus (cucumber) plants. Mitochondrial ultrastructure has been investigated by transmission electron microscopy (TEM) and electron tomography techniques, which allow to obtain a three-dimensional (3D) reconstruction of cellular structures. These analyses revealed that: i) mitochondria appear as crista junction model conformation in cucumber plant and ii) Fe deficiency (-Fe) strongly alters both number and volume of crista when compared to control (+Fe) plants. The ultrastuctural changes observed in Fe-deficient mitochondria reflect a metabolic status characterized by a respiratory chain working at lower rate (orthodox-like conformation) with respect to control mitochondria. To better understand the link between ultrastructure and metabolic status of mitochondria, a biochemical characterization of leaf mitochondria purified from plants grown in the two different Fe nutritional conditions (control and Fe-deficient) has been performed. As far as we know, this is the first report showing a three-dimensional reconstruction of plant mitochondria. Furthermore, these preliminary data together suggest that a detailed charaterization of the link between changes in ultrastructure and functionality of mitochondria during different nutritional conditions can provide a successful approach for understanding the role of these organelles in the plant response to Fe deficiency. [1] Vigani et al., (2013). Trends Plant Sci., 18 : 305-311
TEM tomography reveals a three-dimensional reconstruction of the ultrastructural modifications occurring in Cucumis sativus mitochondria under Fe deficiency / G. Vigani, F. Faoro, A.M. Ferretti, F. Cantele, D. Maffi, M. Marelli, M. Maver, I. Murgia, G. Zocchi. ((Intervento presentato al 43. convegno Meeting of European Society of New methods in Agriculture (ESNA) tenutosi a Bolzano nel 2014.
TEM tomography reveals a three-dimensional reconstruction of the ultrastructural modifications occurring in Cucumis sativus mitochondria under Fe deficiency
G. Vigani
;F. Faoro
;F. Cantele;D. Maffi;M. Marelli;I. MurgiaPenultimo
;G. ZocchiUltimo
2014
Abstract
Mitochondria, as recently suggested [1], might be involved in Fe sensing and signalling pathways in plant cells. For a deep understanding of the role of these organelles in the Fe deficiency-mediating responses in plant cells, it is crucial to provide a full overview of their changes occurring under Fe-limited conditions. The aim of this work is to characterize ultrastructural as well as biochemical changes of leaf mitochondria in Fe-deficient Cucumis sativus (cucumber) plants. Mitochondrial ultrastructure has been investigated by transmission electron microscopy (TEM) and electron tomography techniques, which allow to obtain a three-dimensional (3D) reconstruction of cellular structures. These analyses revealed that: i) mitochondria appear as crista junction model conformation in cucumber plant and ii) Fe deficiency (-Fe) strongly alters both number and volume of crista when compared to control (+Fe) plants. The ultrastuctural changes observed in Fe-deficient mitochondria reflect a metabolic status characterized by a respiratory chain working at lower rate (orthodox-like conformation) with respect to control mitochondria. To better understand the link between ultrastructure and metabolic status of mitochondria, a biochemical characterization of leaf mitochondria purified from plants grown in the two different Fe nutritional conditions (control and Fe-deficient) has been performed. As far as we know, this is the first report showing a three-dimensional reconstruction of plant mitochondria. Furthermore, these preliminary data together suggest that a detailed charaterization of the link between changes in ultrastructure and functionality of mitochondria during different nutritional conditions can provide a successful approach for understanding the role of these organelles in the plant response to Fe deficiency. [1] Vigani et al., (2013). Trends Plant Sci., 18 : 305-311
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