Dendritic cells (DC) have the unique capacities to induce primary T-cell responses. In mice, CD8 alpha+DC are specialized to cross-prime CD8(+) T cells and produce interleukin-12 (IL-12) that promotes cytotoxicity. Human BDCA-3(+) DC share several relevant characteristics with CD8 alpha+DC, but the capacities of human DC subsets to induce CD8(+) T-cell responses are incompletely understood. Here we compared CD1c(+) myeloid DC (mDC) 1, BDCA-3(+)mDC2, and plasmacytoid DC(pDC) in peripheral blood and lymphoid tissues for phenotype, cytokine production, and their capacities to prime cytotoxic T cells. mDC1 were surprisingly the only human DC that secreted high amounts of IL-12p70, but they required combinational Toll-like receptor (TLR) stimulation. mDC2 and pDC produced interferon-lambda and interferon-alpha, respectively. Importantly, mDC1 and mDC2 required different combinations of TLR ligands to cross-present protein antigens to CD8(+) T cells. pDC were inefficient and also expressed lower levels of major histocompatibility complex and co-stimulatory molecules. Nevertheless, all DC induced CD8(+) memory T-cell expansions upon licensing by CD4(+) T cells, and primed naive CD8(+) T cells following appropriate TLR stimulation. However, because mDC1 produced IL-12, they induced the highest levels of cytotoxic molecules. In conclusion, CD1c(+) mDC1 are the relevant source of IL-12 for naive T cells and are fully equipped to cross-prime cytotoxic T-cell responses.
|Titolo:||Human CD1c+ dendritic cells secrete high levels of IL-12 and potently prime cytotoxic T-cell responses|
|Parole Chiave:||antigen cross-presentation; inflamed lymph-nodes; in-vivo; presentation capacity; mouse; subsets; CD8(+); activation; helper; blood|
|Settore Scientifico Disciplinare:||Settore MED/31 - Otorinolaringoiatria|
|Data di pubblicazione:||ago-2013|
|Digital Object Identifier (DOI):||10.1182/blood-2013-04-495424|
|Appare nelle tipologie:||01 - Articolo su periodico|