To study the specificity of cellular MRI based on superparamagnetic iron oxide particles (SPIOs), especially within the CNS. MATERIALS & METHODS: A microglial cell line was engineered for the expression of a suicide gene, the receptor of diphtheria toxin (DT), and two reporter genes, green fluorescent protein and luciferase, in order to induce, in a controlled manner, cell death and test it through bioluminescence. SPIO-labeled DT-sensitive and control DT-insensitive cells were transplanted into the brains of mice, which underwent serial MRI and bioluminescence studies before and up to 90 days after DT-induced cell death. RESULTS: No variations in SPIO signal voids were detected along longitudinal monitoring in brain hemispheres transplanted with DT-sensitive cells. Ex vivo analyses showed persistence of iron nanoparticle deposits at transplantation sites. CONCLUSION: Due to the long-term persistence of signal after transplanted cell death, caution is advised when SPIOs are employed for cell tracking.

Cellular magnetic resonance with iron oxide nanoparticles : long-term persistence of SPIO signal in the CNS after transplanted cell death / C. Cianciaruso, A. Pagani, C. Martelli, M. Bacigaluppi, M. Squadrito, A. Lo Dico, M. De Palma, R. Furlan, G. Lucignani, A. Falini, A. Biffi, L. Ottobrini, L. Politi. - In: NANOMEDICINE. - ISSN 1743-5889. - 9:10(2014 Jul), pp. 1457-1474. [10.2217/nnm.14.84]

Cellular magnetic resonance with iron oxide nanoparticles : long-term persistence of SPIO signal in the CNS after transplanted cell death

C. Martelli;A. Lo Dico;G. Lucignani;L. Ottobrini;
2014-07

Abstract

To study the specificity of cellular MRI based on superparamagnetic iron oxide particles (SPIOs), especially within the CNS. MATERIALS & METHODS: A microglial cell line was engineered for the expression of a suicide gene, the receptor of diphtheria toxin (DT), and two reporter genes, green fluorescent protein and luciferase, in order to induce, in a controlled manner, cell death and test it through bioluminescence. SPIO-labeled DT-sensitive and control DT-insensitive cells were transplanted into the brains of mice, which underwent serial MRI and bioluminescence studies before and up to 90 days after DT-induced cell death. RESULTS: No variations in SPIO signal voids were detected along longitudinal monitoring in brain hemispheres transplanted with DT-sensitive cells. Ex vivo analyses showed persistence of iron nanoparticle deposits at transplantation sites. CONCLUSION: Due to the long-term persistence of signal after transplanted cell death, caution is advised when SPIOs are employed for cell tracking.
cell death; cellular MRI; diphtheria toxin; microglial cell; specificity; superparamagnetic iron oxide
Settore MED/36 - Diagnostica per Immagini e Radioterapia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/246541
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