This report describes the utilization of 111indium-oxine chelate ([111In]Ox) for studies of macrophage-mediated cytotoxicity. [111In]Ox efficiently labeled both non-adherent and adherent tumor targets with no decrease in cell viability. Spontaneous release of intracellularly incorporated [111In]Ox was very slow (0.25-0.50%/h) from most targets, making isotope-release assays of at least 48 h feasible. In addition, released [111In]Ox was not reutilized. In contrast to its low spontaneous release from intact cells, incorporated [111In]Ox was rapidly released from tumor targets after interaction with activated macrophages. Levels of [111In]Ox released in response to cytolytic macrophages correlated well with those observed for the 51Cr and [3H]TdR radiolabels. Therefore, [111In]Ox can be utilized for relatively short-term (less than 20 h) assays with lymphoma targets, as well as for longer-term assays with adherent cells. This should facilitate the testing, with the same radioisotope-release assay, of a wide range of tumor targets for susceptibility to macrophage-mediated cytotoxicity.
|Titolo:||Measurement of macrophage-mediated cytotoxicity against adherent and non-adherent target cells by release of 11 indium-oxine|
TARAMELLI, DONATELLA (Secondo)
|Parole Chiave:||Animals; Cell Adhesion; Cell Survival; Female; Hydroxyquinolines; Indium; Lipopolysaccharides; Macrophages; Mice; Mice, Inbred C57BL; Neoplasms, Experimental; Oxyquinoline; Radioisotopes; Cytotoxicity, Immunologic|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||1981|
|Digital Object Identifier (DOI):||10.1016/0022-1759(81)90180-0|
|Appare nelle tipologie:||01 - Articolo su periodico|