Novel transplantation antigens have been detected in a Moloney virus-induced LSTRA lymphoma of BALB/c origin (H-2d), or in a chemically-induced L5178Y lymphoma of DBA/2 origin, following treatment of tumor-bearing hosts with 5-(3,3′-dimethyl-1-triazeno-)-imidazole-4-carboxamide (DTIC), for 4-8 transplant generations. Marked cell-mediated cytotoxic responses against DTIC-treated LSTRA or L5178Y lines were found by primary in vivo and secondary in vitro sensitization of histocompatible mice. Moreover, preliminary data show that no obvious antigenic cross-reactivity can be found among DTIC-treated sublines derived from distinct parental lymphomas. To test whether DTIC lines would express variable levels of normal histocompatibility antigens, cytotoxic lymphocytes were generated in vitro against alloantigens of H-2 complex or sub-regions of it and tested against parental or DTIC-treated lymphomas in a short-term 51Cr-release assay. A cold-inhibition test was performed with LSTRA or 4 LSTRA/DTIC sublines. The results showed that little or no difference in the expression of H-2 antigens recognized by cytotoxic lymphocytes could be detected between parental and DTIC-treated sublines. Moreover, no foreign H-2 specificities of H-2b or H-2k haplotypes detectable by cytotoxic lymphocytes could have been found in L5178Y or L5178Y/DTIC lymphomas.
Expression of normal histocompatibility antigens in murine lymphomas treated with 5-(3,3'-dimethyl-1-triazeno)-imidazole-4-carboxamide (DTIC) in vivo / D. Taramelli, L. Romani, A. Bonmassar, A. Goldin, M.C. Fioretti. - In: EUROPEAN JOURNAL OF CANCER. - ISSN 0014-2964. - 17:4(1981 Apr), pp. 411-20-420.
|Titolo:||Expression of normal histocompatibility antigens in murine lymphomas treated with 5-(3,3'-dimethyl-1-triazeno)-imidazole-4-carboxamide (DTIC) in vivo|
TARAMELLI, DONATELLA (Primo)
|Parole Chiave:||Animals; Antigens, Neoplasm; Cell Line; Cross Reactions; Cytotoxicity, Immunologic; Dacarbazine; Epitopes; Female; H-2 Antigens; Leukemia L5178; Leukemia, Experimental; Male; Mice; Mice, Inbred Strains; Moloney murine leukemia virus|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||apr-1981|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0014-2964(81)90250-4|
|Appare nelle tipologie:||01 - Articolo su periodico|