A subpopulation of scleroderma dermal fibroblasts was identified by flow cytometric analysis. Between 15% and 25% of the cells within the scleroderma fibroblast lines had high levels of cytoplasmic granularity, as identified by side light scatter characteristics. Similar fibroblasts composed less than 3% of the cells within the normal fibroblast lines, although greater numbers could be induced through exposure to soluble factors derived from activated mononuclear cells. The granular subpopulation of fibroblasts produced 2-3 times as much procollagen as did the other fibroblasts. These data support the hypothesis that fibrosis in scleroderma may result in part from the activity of an inherently high procollagen-producing subset of normal fibroblasts that is expanded through exposure to immune cytokines.
|Titolo:||In vitro identification of a subpopulation of fibroblasts that produces high levels of collagen in scleroderma patients|
|Parole Chiave:||adult; biological factors; cell line; collagen; cytokines; fibroblasts; flow cytometry; humans; middle aged; monocytes; procollagen; protein biosynthesis; reference values; reproducibility of results; scleroderma, localized; skin|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||giu-1990|
|Digital Object Identifier (DOI):||10.1002/art.1780330611|
|Appare nelle tipologie:||01 - Articolo su periodico|