Hemozoin, the detoxification product of hemoglobin heme, piles up as electron-dense material in the food vacuole (FV) of intraerythrocytic malaria parasites (malaria pigment). In infected individuals, pigment is internalized by both circulating and resident phagocytes, thus modulating their functions. Synthetic beta-hematin, prepared in vitro from hematin (ferriprotoporphyrin IX hydroxide) in acidic condition, is spectroscopically identical to hemozoin. In this electron microscopy study, native and synthetic hemozoin also prove to be morphologically indistinguishable (large polygonal crystals with apparent transverse banding) and to undergo the same process when internalized by phagocytes (primarily a direct uptake of crystals, similar to what is described for asbestos fibers). On the contrary, whole parasites appear to follow a classical endocytic pathway. This suggests that there may be differences between the ingestion of free particles and whole parasites in terms of modulation of phagocytes' functions.
|Titolo:||Phagocytosis of hemozoin (native and synthetic malaria pigment), and Plasmodium falciparum intraerythrocyte-stage parasites by human and mouse phagocytes|
TARAMELLI, DONATELLA (Penultimo)
|Parole Chiave:||animals; cell line; erythrocytes; hemeproteins; humans; macrophages; mice; microscopy, electron; monocytes; pigments, biological; Plasmodium falciparum; phagocytosis; β-hematin; hemozoin; malaria; malaria pigment; phagocytosis; red blood cells|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||gen-2000|
|Digital Object Identifier (DOI):||10.1080/019131200281264|
|Appare nelle tipologie:||01 - Articolo su periodico|