This paper reports the characterization of an electrochemical biosensor for the continuous monitoring of Naproxen based on cytochrome P450. The electrochemical biosensor is based on the drop-casting of multi-walled carbon-nanotubes (MWCNTs) and microsomal cytochrome P4501A2 (msCYP1A2) on a graphite screen-printed electrode (SPE).The proposed biosensor was employed to monitor Naproxen (NAP), a well-known anti-inflammatory compound, through cyclic voltammetry. The dynamic linear range for the amperometric detection of NAP had an upper limit of 300. μM with a corresponding limit of detection (LOD) of 16±1. μM (S/N=3), which is included in NAP physiological range (9-300. μM). The MWCNT/msCYP1A2-SPE sensor was also calibrated for NAP detection in mouse serum that was previously extracted from mice, showing a slightly higher LOD (33±18. μM).The stability of the msCYP1A2-based biosensor was assessed by longtime continuous cyclic voltammetric measurements. The ability of the sensor to monitor drug delivery was investigated by using a commercial micro-osmotic pump. Results show that the MWCNT/msCYP1A2-SPE sensor is capable of precisely monitoring the real-time delivery of NAP for 16. h. This work proves that the proposed electrochemical sensor might represent an innovative point-of-care solution for the personalization of drug therapies, as well as for pharmacokinetic studies in both animals and humans.© 2013 Elsevier B.V.
|Titolo:||Continuous monitoring of Naproxen by a cytochrome P450-based electrochemical sensor|
|Parole Chiave:||Carbon nanotubes ; Continuous drug monitoring ; Cytochrome P450 ; Electrochemical biosensors ; Personalized therapy ; Biophysics ; Biomedical Engineering ; Biotechnology ; Electrochemistry|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Data di pubblicazione:||15-mar-2014|
|Digital Object Identifier (DOI):||10.1016/j.bios.2013.09.058|
|Appare nelle tipologie:||01 - Articolo su periodico|