Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.

Customized patterned substrates for highly versatile correlative light-scanning electron microscopy / L. Benedetti, E. Sogne, S. Rodighiero, D. Marchesi, P. Milani, M. Francolini. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 4(2014 Nov 13), pp. 7033.1-7033.5. [10.1038/srep07033]

Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

L. Benedetti
Primo
;
E. Sogne
Secondo
;
S. Rodighiero;P. Milani
Penultimo
;
M. Francolini
Ultimo
2014

Abstract

Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.
CLEM; scanning electron microscopy; confocal microscopy; patterned substrates
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/244621
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