The characterization of grapevine genetic resources in the Caucasian and Eastern European regions is one of the main goals of the COST Action FA1003 European project to preserve their genetic heritage and to improve the knowledge about genetic variability of V. vinifera in the countries considered to be the birth place for viticulture like the South Caucasus. Ten SSR loci were used to characterize the autochthonous grapevine cultivars from Armenia (29), Azerbaijan (46), Georgia (44), Moldova (23) and 22 central European cultivars for comparison. The SSR allelic profiles were analysed by GenAlEx 4.2 software to investigate the genetic diversity and STRUCTURE 3.2 software to investigate the admixture proportions among the germplasms. The statistical analysis identified Azerbaijan germplasm having the highest genetic diversity. The first two coordinates of PCA, accounted for about 51% of total variability, separated the genotypes in two main groups with clear overlapping areas, the Azerbaijan group and the Caucasian and European group. The STRUCTURE analysis revealed three main genetic groups (K=3): i) central Europe genotypes; ii) admixed group including Armenian, Georgian and Moldova cultivars; iii) Azerbaijan cultivars. The analysis of genetic relationships in our dataset provided evidence of connection among cultivars included in the proles pontica and proles orientalis. Moreover, the high genetic diversity of the South Caucasian germplasm with respect to other European germplams was in agreement with the consideration of this region as the cradle for grapevine domestication.
|Titolo:||Molecular investigation of caucasian and eastern european grapevine genetic resources (V. vinifera L.) by microsatellites|
DE LORENZIS, GABRIELLA (Corresponding)
BIAGINI, BARBARA SARA (Secondo)
|Parole Chiave:||Genotyping; Relationship; SSR; Vitis vinifera|
|Settore Scientifico Disciplinare:||Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree|
|Data di pubblicazione:||2014|
|Appare nelle tipologie:||01 - Articolo su periodico|