Background aims. In attempting to develop new strategies to circumvent the immunosuppression associated with glioblastoma (GB), novel approaches have been designed using dendritic cell (DC)-based vaccination, which is considered a promising strategy to attack high-grade glioma. In previous studies, we demonstrated that human mesenchymal stromal cells without genetic manipulation but primed with Paclitaxel (PTX) acquire a potent anti-tumor activity, providing an interesting new biological approach for drug delivery. On the basis of these results, we here investigated whether both CD14+ and their derived DCs may behave like mesenchymal stromal cells acquiring anti-tumor activity on priming with PTX. Methods. Human CD14+ cells were isolated from peripheral blood. Fluorescence-activated cell sorter analysis, was performed to determine the purity of CD14+ and their differentiation into mature DCs. Cells were primed by incubation with 1 mu g/mL of PTX for 24 h, and the PTX released by cells was assessed by mass spectrometry analysis. Anti-tumor activity was checked by testing the conditioned medium (CM) on the proliferation of U87 MG, a GB cell line. Results. Both CD14+ and DCs were able to incorporate PTX and release the drug in the CM in a time-dependent manner (maximal release over 24 h). The addition of CM from CD14+ and DCs loaded with PTX strongly inhibits proliferation of U87 MG cells. Conclusions. Our results are the first demonstration that peripheral blood-derived CD14+ and DCs, in addition to their application for immunotherapy for GB, could also be used to delivery anti-cancer drugs, such as PTX, to kill GB cells.
Human CD14+ cells loaded with Paclitaxel inhibit in vitro cell proliferation of glioblastoma / A. Bonomi, D. Lisini, S. E. Navone, S. Frigerio, M. Dossena, E. Ciusani, P. Rampini, G. Marfia, V. Coccè, L. Cavicchini, F. Sisto, E. Parati, R. Mantegazza, M. Rimoldi, M. Rizzetto, G. Alessandri, A. Pessina. - In: CYTOTHERAPY. - ISSN 1465-3249. - 17:3(2015), pp. 310-319.
|Titolo:||Human CD14+ cells loaded with Paclitaxel inhibit in vitro cell proliferation of glioblastoma|
BONOMI, ARIANNA (Primo)
PESSINA, AUGUSTO (Corresponding)
|Parole Chiave:||CD14+cells; drug uptake; glioblastoma; Taxol|
|Settore Scientifico Disciplinare:||Settore MED/07 - Microbiologia e Microbiologia Clinica|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jcyt.2014.09.009|
|Appare nelle tipologie:||01 - Articolo su periodico|