IL-13 has been implicated in the pathogenesis of ulcerative colitis (UC), and may have a role in animal models of gut fibrosis. We studied the involvement of IL-13 in inflammation and fibrosis in UC and Crohn's disease (CD). Intestinal biopsies and anti-CD3/CD28- or anti-CD2/CD28-stimulated lamina propria mononuclear cells from UC and CD patients and control subjects were cultured, and IL-13, IL-4, IL-5, IL-17A and IFN-γ production was measured. Mucosal IL-13-producing cells were characterised by flow cytometry. Gut explants from strictured CD, non-strictured CD and healthy donors were cultured ex vivo, and secreted IL-13, IL-1β and collagen were measured. IL-13 production by mucosal explants and activated lamina propria mononuclear cells did not differ between CD, UC and control subjects, and was at least a log lower than IFN-γ and IL-17A. IL-13-producing cells, and in particular natural killer T cells, were uniformly low in all groups. IL-4 and IL-5 were undetectable in culture supernatants. Explants of CD strictures produced low amounts of IL-13, whereas IL-1β and collagen were elevated. We could not confirm that UC or strictured CD are associated with elevated IL-13 production. These data suggest that an anti-IL-13 Ab would not be an appropriate therapeutic strategy in inflammatory bowel disease. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
|Titolo:||Absence of a role for interleukin-13 in inflammatory bowel disease|
|Parole Chiave:||Crohn's disease ; Fibrosis ; T helper cell type 2 ; Ulcerative colitis ; Adolescent ; Adult ; Aged ; Antigens, CD28 ; Antigens, CD3 ; Colitis, Ulcerative ; Crohn Disease ; Fibrosis ; Humans ; Inflammation ; Inflammatory Bowel Diseases ; Interferon-gamma ; Interleukin-13 ; Interleukin-13 Receptor alpha1 Subunit ; Interleukin-13 Receptor alpha2 Subunit ; Intestinal Mucosa ; Intestines; Leukocytes, Mononuclear; Macrophages ; Middle Aged ; Mucous Membrane ; Natural Killer T-Cells ; Th2 Cells ; Transforming Growth Factor beta1 ; Young Adult ; Immunology ; Immunology and Allergy|
|Settore Scientifico Disciplinare:||Settore MED/12 - Gastroenterologia|
|Data di pubblicazione:||feb-2014|
|Digital Object Identifier (DOI):||10.1002/eji.201343524|
|Appare nelle tipologie:||01 - Articolo su periodico|