Elongation factor EF1 was found in a low salt homogenate of wheat embryos, either in the 100 000 X g supernatant or in the ribosome pellet. The ribosome-linked EF1 (EF1R), deteched by high salt washing, was purified to electrophoretical homogenetiy and its molecular and functional properties compared to those of a purified high molecular weight species of EF1 obtained from cytoplasm (EF1H). The two forms are associations of different polypeptides having in common only the polypeptide which can form the ternary complex with aminoacyl-tRNA and GTP. Whereas EF1R is able to fulfill all the EF1 functions, EF1H, incubated with ribosomes completely deprived of elongation factors, can catalyze the aminoacyl-tRNA binding to ribosomes, but, in the presence of EF2, forms only a very small amount of poly(Phe).

Subcellular distribution and functional properties of different forms of elongation factor EF1 from wheat embryos / G.A. Lanzani, E. Caldiroli, G. Zocchi, L.A. Manzocchi, R. Bollini, L. De Alberti. - In: BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE. - ISSN 0005-2795. - 407:4(1975 Nov), pp. 449-458.

Subcellular distribution and functional properties of different forms of elongation factor EF1 from wheat embryos

G. Zocchi;
1975

Abstract

Elongation factor EF1 was found in a low salt homogenate of wheat embryos, either in the 100 000 X g supernatant or in the ribosome pellet. The ribosome-linked EF1 (EF1R), deteched by high salt washing, was purified to electrophoretical homogenetiy and its molecular and functional properties compared to those of a purified high molecular weight species of EF1 obtained from cytoplasm (EF1H). The two forms are associations of different polypeptides having in common only the polypeptide which can form the ternary complex with aminoacyl-tRNA and GTP. Whereas EF1R is able to fulfill all the EF1 functions, EF1H, incubated with ribosomes completely deprived of elongation factors, can catalyze the aminoacyl-tRNA binding to ribosomes, but, in the presence of EF2, forms only a very small amount of poly(Phe).
Settore AGR/13 - Chimica Agraria
nov-1975
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/240509
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