Brief cold shock given to corn root tissue which has been preloaded with 32P-labeled phosphate increases the phosphorylation of microsomal membrane proteins. Gel electrophoresis shows that discrete proteins are labeled. Preincubation of sealed microsomal vesicles under conditions producing membrane protein phosphorylation causes a 25% loss of total ATPase activity and 55% loss of uncoupler-sensitive activity. It is believed that the effect of injury or shock in blocking H+ efflux pumping by corn roots may be attributed to phosphorylation of the H+-ATPase. Calcium influx attending injury may be responsible for activating the protein kinase.
|Titolo:||Inhibition of proton pumping in corn roots is associated with increased phosphorylation of membrane proteins|
ZOCCHI, GRAZIANO (Corresponding)
|Parole Chiave:||Calcium; Calmodulin; H+-ATPase; Microsomal membranes; Protein phosphorylation|
|Settore Scientifico Disciplinare:||Settore AGR/13 - Chimica Agraria|
|Data di pubblicazione:||set-1983|
|Digital Object Identifier (DOI):||10.1016/0304-4211(83)90058-5|
|Appare nelle tipologie:||01 - Articolo su periodico|