Cucumber (Cucumis sativus L.) is considered a model plant for the investigation of Fe deficiency responses, since it strongly exhibits typical strategy I, i. e. increased activities of Fe(III)-chelate reductase, H +-ATPase and iron regulated transporters. In this study, cDNA amplified fragment length polymorphism analysis was employed to identify genes differentially expressed in the root apex following Fe deficiency. The expression patterns of the most interesting transcript derived fragments were validated by semiquantitative reverse trascriptase - polymerase chain reaction. A set of new genes overexpressed under Fe deficiency, such as those coding for calmodulin, SNAP, TIM23 and V-PPase were identified. Furthermore, we also observed that calmodulin protein accumulated in Fe-deficient root apexes.

cDNA-AFLP analysis reveals a set of new genes differentially expressed in cucumber root apexes in response to iron deficiency / G. Vigani, A. Chittò, P. De Nisi, G. Zocchi. - In: BIOLOGIA PLANTARUM. - ISSN 0006-3134. - 56:3(2012 Sep), pp. 502-508. [10.1007/s10535-012-0050-1]

cDNA-AFLP analysis reveals a set of new genes differentially expressed in cucumber root apexes in response to iron deficiency

G. Vigani;P. De Nisi;G. Zocchi
2012-09

Abstract

Cucumber (Cucumis sativus L.) is considered a model plant for the investigation of Fe deficiency responses, since it strongly exhibits typical strategy I, i. e. increased activities of Fe(III)-chelate reductase, H +-ATPase and iron regulated transporters. In this study, cDNA amplified fragment length polymorphism analysis was employed to identify genes differentially expressed in the root apex following Fe deficiency. The expression patterns of the most interesting transcript derived fragments were validated by semiquantitative reverse trascriptase - polymerase chain reaction. A set of new genes overexpressed under Fe deficiency, such as those coding for calmodulin, SNAP, TIM23 and V-PPase were identified. Furthermore, we also observed that calmodulin protein accumulated in Fe-deficient root apexes.
calmodulin ; Cucumis sativus ; semiquantitative RT-PCR ; transcript-derived fragments ; transcriptomic analysis
Settore AGR/13 - Chimica Agraria
Article (author)
File in questo prodotto:
File Dimensione Formato  
art%3A10.1007%2Fs10535-012-0050-1.pdf

accesso riservato

Descrizione: ArticoloZocchiBiologiaplantarum
Tipologia: Publisher's version/PDF
Dimensione 439.71 kB
Formato Adobe PDF
439.71 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/240427
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 8
social impact