Detergent resistant membranes (DRMs) are a useful model system for the in vitro characterization of cell membrane domains. Indeed, DRMs provide a simple model to study the mechanisms underlying several key cell processes based on the interplay between specific cell membrane domains on one hand, and specific proteins and/or lipids on the other. Considering therefore their biological relevance, the development of methods capable to provide information on the composition and structure of membrane domains and to detect their modifications is highly desirable. In particular, Fourier transform infrared (FTIR) spectroscopy is a vibrational tool widely used for the study not only of isolated and purified biomolecules but also of complex biological systems, including intact cells and tissues. One of the main advantages of this non-invasive approach is that it allows obtaining a molecular fingerprint of the sample under investigation in a rapid and label-free way.

A Fourier transform infrared spectroscopy study of cell membrane domain modifications induced by docosahexaenoic acid / P. Mereghetti, P.A. Corsetto, A. Cremona, A.M. Rizzo, S.M. Doglia, D. Ami. - In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS. - ISSN 0304-4165. - 1840:10(2014 Oct), pp. 3115-3122. [10.1016/j.bbagen.2014.07.003]

A Fourier transform infrared spectroscopy study of cell membrane domain modifications induced by docosahexaenoic acid

P.A. Corsetto
Primo
;
A. Cremona;A.M. Rizzo
Penultimo
;
2014-10

Abstract

Detergent resistant membranes (DRMs) are a useful model system for the in vitro characterization of cell membrane domains. Indeed, DRMs provide a simple model to study the mechanisms underlying several key cell processes based on the interplay between specific cell membrane domains on one hand, and specific proteins and/or lipids on the other. Considering therefore their biological relevance, the development of methods capable to provide information on the composition and structure of membrane domains and to detect their modifications is highly desirable. In particular, Fourier transform infrared (FTIR) spectroscopy is a vibrational tool widely used for the study not only of isolated and purified biomolecules but also of complex biological systems, including intact cells and tissues. One of the main advantages of this non-invasive approach is that it allows obtaining a molecular fingerprint of the sample under investigation in a rapid and label-free way.
ATR-FTIR spectroscopy; cholestero l; DHA; detergent resistant membranes; multidimensional scaling; sphingomyelin
Settore BIO/10 - Biochimica
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/240145
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