Artemisinin is a sesquiterpene lactone endoperoxide, extracted from Artemisia annua. Beside antimalarial property, artemisinin and its analogues have been shown to possess immunomodulatory and antitumor effects. Recently, it was reported that artemisinin and a series of new derivates, are also active against Helicobater pylori. The aim of this study was to evaluate the antibacterial activity of artemisone, a new semi synthetic derivative of artemisinin, against both susceptible and resistant strains of Helicobacter pylori isolated in Lombardia region, Italy. Artemisone is a second-generation artemisinin that has shown improved pharmacokinetic properties including longer half-life and lower toxicity. The compound was tested against 25 clinical isolates and one reference strain of H. pylori using the broth microdilution methodology. Furthermore, in order to verify the rate and extent of killing of H. pylori by the artemisone, bactericidal kinetic was also performed against the reference strain ATCC 43504. The inhibitory concentrations of artemisone were similar to the bactericidal ones, with values ranging from 0.25 to 0.5 μg/ml. Independently from their susceptibility to metronidazole or chlaritromycin, 90% of the investigated strains were inhibited in their growth at 0.5 μg/ml. A decrease of 4-log10 in cell count was observed after 48 h at 0.5 μg/ml. These results show that artemisone has a promising antibacterial properties against H. pylori and, therefore, in combination with antibiotics, could represent a novel strategy for the treatment of the infection especially in cases of multiresistance.
In vitro activity of artemisone against different strains of Helicobacter pylori / F. Sisto, M.M. Scaltrito, C. Masia, A.K. Parvez, R.K. Haynes, D. Taramelli. - In: HELICOBACTER. - ISSN 1083-4389. - 19:suppl. 1(2014 Sep), pp. 148-148. ((Intervento presentato al 27. convegno International Workshop of the European Helicobacter Study Group tenutosi a Roma nel 2014.
|Titolo:||In vitro activity of artemisone against different strains of Helicobacter pylori|
SISTO, FRANCESCA (Corresponding)
SCALTRITO, MARIA MADDALENA (Secondo)
TARAMELLI, DONATELLA (Ultimo)
|Settore Scientifico Disciplinare:||Settore MED/07 - Microbiologia e Microbiologia Clinica|
Settore MED/04 - Patologia Generale
|Data di pubblicazione:||set-2014|
|Enti collegati al convegno:||European Helicobacter Study Group|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/hel.12166|
|Appare nelle tipologie:||01 - Articolo su periodico|