Spinal and bulbar muscular atrophy (SBMA) and Amyotrophic lateral sclerosis (ALS) are two adult onset motoneuron disease. Different mutant proteins have been found involved in these diseases (e.g.: androgen receptor (AR), SOD1 and TDP43). These proteins tend to acquire aberrant conformations (misfolding) that lead to their aggregation. Aggregates may be protective by subtracting misfolded proteins, but may also impair proteasome and autophagy, two major players of the protein quality control system (PQC). We recently showed that HSPB8 mediated autophagy reduces misfolded proteins aggregation, even when proteasome is inhibited. Dynein by binding BAG3 and HSPB8, transports misfolded proteins to microtubule organization center (MTOC), where proteins are assembled in aggresome to be degraded by autophagy. Dynein also mediates autophagosome formation, by binding Ambra1-Beclin1-Vps34 complex that enable autophagosome nucleation. In SBMA cell model we found that dynein is sequestered into aggregates of mutant AR near MTOC. To evaluate the role of dynein SBMA and ALS we used EHNA, an inhibitor of dynein ATPase activity witch impairs dynein-mediated transport. In cell model of SBMA and ALS EHNA induced a drastic reduction of aggregates of the misfolded proteins ARpolyQ, mutSOD1, and mutTDP43. EHNA, also, increased the solubility of misfolded ARpolyQ. BAG1, witch routes misfolding proteins to proteasome degradation, was found increased after EHNA treatment. Our data are in line with previous observation that showing an increase of survival of the double mutant mice for mutSOD1 and an inactive form of dynein. This suggests that dynein may mediates aggregates formation process during the PQC response. GRANTS: Telethon; Fondazione AriSLA; AFM, France; Fondation Thierry Latran, France; Regione Lombardia; Università degli Studi di Milano.
Inhibition of dynein ATPase activity reduces aggregates formation of misfolded proteins involved in motoneuron diseases / R. Cristofani, E. Giorgetti, V.Crippa, A. Boncoraglio, P. Rusmini, A. Poletti. ((Intervento presentato al 9. convegno FENS Forum of neuroscience tenutosi a Milano nel 2014.
|Titolo:||Inhibition of dynein ATPase activity reduces aggregates formation of misfolded proteins involved in motoneuron diseases|
CRISTOFANI, RICCARDO MARIA (Primo)
|Data di pubblicazione:||6-lug-2014|
|Parole Chiave:||ALS ; SBMA ; Dynein|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Citazione:||Inhibition of dynein ATPase activity reduces aggregates formation of misfolded proteins involved in motoneuron diseases / R. Cristofani, E. Giorgetti, V.Crippa, A. Boncoraglio, P. Rusmini, A. Poletti. ((Intervento presentato al 9. convegno FENS Forum of neuroscience tenutosi a Milano nel 2014.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|