The preparation of nucleosides as well as their base-modified analogues using purified nucleoside phosphorylase enzymes or, more conveniently, using whole bacterial cells is described. The development of genetically modified strains of Escherichia coli, able to over-produce Uridine-phosphorylase and Purine-nucleoside-phosphorylase in the same cells, improves the specific biocatalytic activity and the consequent industrial scale approach.
Recombinant bacterial cells as efficient biocatalysts for the production of nucleosides / E. Spoldi, D. Ghisotti, S. Cali', M. Grisa, G. Tonon, G. Orsini, G. Zuffi. - In: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS. - ISSN 1525-7770. - 20:4-7(2001), pp. 977-979.
Recombinant bacterial cells as efficient biocatalysts for the production of nucleosides
D. Ghisotti;
2001
Abstract
The preparation of nucleosides as well as their base-modified analogues using purified nucleoside phosphorylase enzymes or, more conveniently, using whole bacterial cells is described. The development of genetically modified strains of Escherichia coli, able to over-produce Uridine-phosphorylase and Purine-nucleoside-phosphorylase in the same cells, improves the specific biocatalytic activity and the consequent industrial scale approach.
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